Identification of a human endogenous LTR-like sequence using HIV-1 LTR specific primers

Chiara Menin, Stefano Indraccolo, Marco Montagna, Barbara Corneo, Laura Bonaldi, Christine Leib-Mösch, Luigi Chieco-Bianchi, Emma D'Andrea

Research output: Contribution to journalArticlepeer-review

Abstract

Using 'consensus' primers derived from the LTR region of 15 HIV-1 isolates, a fragment of 583 bp was amplified from human DNA. Even though specificity was confirmed by Southern blot analysis with a conserved LTR oligonucleotide probe, no significant homologies were detected to either retroviral regions or human or non-human published sequences. Nevertheless, when used as a probe, the 583-bp fragment identified a unique DNA sequence in the human genome on chromosome 1, and cross-reactive sequences in monkey, but not mouse, DNA. This novel, unique and conserved sequence of 583 bp was used to isolate a human HS-1 clone in which the structural property of a viral LTR could be identified.

Original languageEnglish
Pages (from-to)443-451
Number of pages9
JournalMolecular and Cellular Probes
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 1996

Keywords

  • Conserved human DNA
  • HIV-1
  • LTR consensus primers
  • Unique LTR-like sequence

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

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