Identification of a new control region in the genome of the DDP strain of BK virus isolated from PBMC

Anna Marta Degener, Valeria Pietropaolo, Cristiana Di Taranto, Li Jin, Franco Ameglio, Paola Cordiali-Fei, Elisabetta Trento, Laura Sinibaldi, Nicola Orsi

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Abstract

The various strains of human polyomavirus BK (BKV) show a marked heterogeneity in the noncoding control region (NCCR), which includes the origin of replication and the regulatory region for early and late transcription. A new BKV strain (DDP, U91605) was identified by direct detection and sequencing of PCR products of BKV-NCCR DNA obtained from PBMC samples of HIV-positive or -negative subjects. The DDP strain NCCR sequence showed an organisation not described previously in vivo with the maximum homology with the archetypal strain (WW) (M34048), as compared with those collected in GenBank. Structurally, P68, Q39, and S68 boxes were perfectly conserved, whereas the R63 box was completely deleted. This deletion involves the loss of sequences able to bind cellular factors essential for the DNA transcription, such as NF1 binding sites, normally present twice in the R box and the modification of SP1. It is possible that these rearrangements represent a cause of the loss of the VP1 region observed in 9/22 PBMC samples and never observed in urine isolates, which are similar to the WW strain.

Original languageEnglish
Pages (from-to)413-419
Number of pages7
JournalJournal of Medical Virology
Volume58
Issue number4
DOIs
Publication statusPublished - 1999

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Keywords

  • Binding sites
  • BKV
  • DDP strain
  • Human polyomavirus
  • NCCR
  • Transcriptional factors

ASJC Scopus subject areas

  • Virology

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