Abstract
The various strains of human polyomavirus BK (BKV) show a marked heterogeneity in the noncoding control region (NCCR), which includes the origin of replication and the regulatory region for early and late transcription. A new BKV strain (DDP, U91605) was identified by direct detection and sequencing of PCR products of BKV-NCCR DNA obtained from PBMC samples of HIV-positive or -negative subjects. The DDP strain NCCR sequence showed an organisation not described previously in vivo with the maximum homology with the archetypal strain (WW) (M34048), as compared with those collected in GenBank. Structurally, P68, Q39, and S68 boxes were perfectly conserved, whereas the R63 box was completely deleted. This deletion involves the loss of sequences able to bind cellular factors essential for the DNA transcription, such as NF1 binding sites, normally present twice in the R box and the modification of SP1. It is possible that these rearrangements represent a cause of the loss of the VP1 region observed in 9/22 PBMC samples and never observed in urine isolates, which are similar to the WW strain.
| Original language | English |
|---|---|
| Pages (from-to) | 413-419 |
| Number of pages | 7 |
| Journal | Journal of Medical Virology |
| Volume | 58 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1999 |
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Keywords
- Binding sites
- BKV
- DDP strain
- Human polyomavirus
- NCCR
- Transcriptional factors
ASJC Scopus subject areas
- Virology
Cite this
Identification of a new control region in the genome of the DDP strain of BK virus isolated from PBMC. / Degener, Anna Marta; Pietropaolo, Valeria; Di Taranto, Cristiana; Jin, Li; Ameglio, Franco; Cordiali-Fei, Paola; Trento, Elisabetta; Sinibaldi, Laura; Orsi, Nicola.
In: Journal of Medical Virology, Vol. 58, No. 4, 1999, p. 413-419.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of a new control region in the genome of the DDP strain of BK virus isolated from PBMC
AU - Degener, Anna Marta
AU - Pietropaolo, Valeria
AU - Di Taranto, Cristiana
AU - Jin, Li
AU - Ameglio, Franco
AU - Cordiali-Fei, Paola
AU - Trento, Elisabetta
AU - Sinibaldi, Laura
AU - Orsi, Nicola
PY - 1999
Y1 - 1999
N2 - The various strains of human polyomavirus BK (BKV) show a marked heterogeneity in the noncoding control region (NCCR), which includes the origin of replication and the regulatory region for early and late transcription. A new BKV strain (DDP, U91605) was identified by direct detection and sequencing of PCR products of BKV-NCCR DNA obtained from PBMC samples of HIV-positive or -negative subjects. The DDP strain NCCR sequence showed an organisation not described previously in vivo with the maximum homology with the archetypal strain (WW) (M34048), as compared with those collected in GenBank. Structurally, P68, Q39, and S68 boxes were perfectly conserved, whereas the R63 box was completely deleted. This deletion involves the loss of sequences able to bind cellular factors essential for the DNA transcription, such as NF1 binding sites, normally present twice in the R box and the modification of SP1. It is possible that these rearrangements represent a cause of the loss of the VP1 region observed in 9/22 PBMC samples and never observed in urine isolates, which are similar to the WW strain.
AB - The various strains of human polyomavirus BK (BKV) show a marked heterogeneity in the noncoding control region (NCCR), which includes the origin of replication and the regulatory region for early and late transcription. A new BKV strain (DDP, U91605) was identified by direct detection and sequencing of PCR products of BKV-NCCR DNA obtained from PBMC samples of HIV-positive or -negative subjects. The DDP strain NCCR sequence showed an organisation not described previously in vivo with the maximum homology with the archetypal strain (WW) (M34048), as compared with those collected in GenBank. Structurally, P68, Q39, and S68 boxes were perfectly conserved, whereas the R63 box was completely deleted. This deletion involves the loss of sequences able to bind cellular factors essential for the DNA transcription, such as NF1 binding sites, normally present twice in the R box and the modification of SP1. It is possible that these rearrangements represent a cause of the loss of the VP1 region observed in 9/22 PBMC samples and never observed in urine isolates, which are similar to the WW strain.
KW - Binding sites
KW - BKV
KW - DDP strain
KW - Human polyomavirus
KW - NCCR
KW - Transcriptional factors
UR - http://www.scopus.com/inward/record.url?scp=0032770543&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032770543&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1096-9071(199908)58:4<413::AID-JMV15>3.0.CO;2-W
DO - 10.1002/(SICI)1096-9071(199908)58:4<413::AID-JMV15>3.0.CO;2-W
M3 - Article
VL - 58
SP - 413
EP - 419
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 4
ER -