Identification of a novel antiviral micro-RNA targeting the NS1 protein of the H1N1 pandemic human influenza virus and a corresponding viral escape mutation

Laura Bavagnoli, Giulia Campanini, Maurizio Forte, Giorgia Ceccotti, Elena Percivalle, Silvia Bione, Antonella Lisa, Fausto Baldanti, Giovanni Maga

Research output: Contribution to journalArticle

Abstract

The influenza A virus (IAV) NS1 protein is one of the major regulators of pathogenicity, being able to suppress innate immune response and host protein synthesis. In this study we identified the human micro RNA hsa-miR-1307-3p as a novel potent suppressor of NS1 expression and influenza virus replication. Transcriptomic analysis indicates that hsa-miR-1307-3p also negatively regulates apoptosis and promotes cell proliferation. In addition, we identified a novel mutation in the NS1 gene of A(H1N1)pdm09 strains circulating in Italy in the 2010-11 season, which enabled the virus to escape the hsa-miR-1307-3p inhibition, conferring replicative advantage to the virus in human cells. To the best of our knowledge, this is the first validation of suppression of IAV H1N1 NS1 by a human micro RNA and the first example of an escape mutation from micro RNA-mediated antiviral response for the A(H1N1)pdm09 virus.

Original languageEnglish
Article number104593
JournalAntiviral Research
Volume171
DOIs
Publication statusPublished - Nov 1 2019

Keywords

  • Antiviral micro-RNA
  • Escape mutation
  • Host-pathogen interaction
  • Human influenza a virus
  • Influenza pandemics

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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