Identification of a novel domain of HIV tat involved in monocyte chemotaxis

Adriana Albini, Roberto Benelli, Daniela Giunciuglio, Tania Cai, Giuliano Mariani, Silvano Ferrini, Douglas M. Noonan

Research output: Contribution to journalArticlepeer-review

Abstract

Human immunodeficiency virus (HIV) Tat is chemotactic for monocytes and dendritic cells, an activity that could play a key role in the expansion of HIV infection of accessory cells. To date, domains of Tat previously found to interact with cell surface molecules have shown only partial chemotactic activity toward monocytes. Using overlapping Tat peptides, we identify a novel region of Tat with a potent chemotactic activity for monocytes, reaching levels equal to Tat itself. This peptide also provokes monocyte polarization similar to Tat and is able to compete with Tat for induction of monocyte migration. Specific high affinity (k(d) = 3 x 10-9 M) cell surface binding sites on monocyte cell surfaces for this region of Tat are demonstrated. These data indicate that the majority of Tat effects on monocytes are mediated by a novel region in the cysteine-rich and core domains. These domains are highly conserved among different HIV isolates, suggesting an important role in the establishment of HIV infection.

Original languageEnglish
Pages (from-to)15895-15900
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number26
DOIs
Publication statusPublished - Jun 26 1998

ASJC Scopus subject areas

  • Biochemistry

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