TY - JOUR
T1 - Identification of a novel interleukin-15 (IL-15) transcript isoform generated by alternative splicing in human small cell lung cancer cell lines
AU - Meazza, Raffaella
AU - Verdiani, Simonetta
AU - Biassoni, Roberto
AU - Coppolecchia, Massimiliano
AU - Gaggero, Alessia
AU - Orengo, Anna Maria
AU - Colombo, Mario P.
AU - Azzarone, Bruno
AU - Ferrini, Silvano
PY - 1996
Y1 - 1996
N2 - IL-15 is a cytokine promoting growth and differentiation of T, B and NK lymphocytes. By RT-PCR analysis, using primers allowing amplification of the entire IL-15 mRNA coding region, 9/11 small cell lung cancer (SCLC) cell lines displayed detectable IL-15 gene expression. In addition to the expected band sizing 524 bp, a larger band was also observed. Cloning and sequence analysis of the larger cDNA from two SCLC cell lines revealed a size of 643 bp due to the presence of additional 119 bp within the previously reported IL-15 cDNA sequence. The 119 bp sequence matched with an IL-15 genomic sequence downstream the IL-15 second coding exon and may represent a previously unreported alternative exon (exon A). The SCLC-associated IL-15 mRNA isoform has a shorter open reading frame (ORF) due to stop codons in exon A, followed by a new AUG codon. The predicted IL-15 precursor protein displays a shorter signal peptide but shares the same aminoacidic composition of mature IL-15 protein. A possible functional role of IL-15, different from 'IL-2-like' activity, in human tumours, is suggested.
AB - IL-15 is a cytokine promoting growth and differentiation of T, B and NK lymphocytes. By RT-PCR analysis, using primers allowing amplification of the entire IL-15 mRNA coding region, 9/11 small cell lung cancer (SCLC) cell lines displayed detectable IL-15 gene expression. In addition to the expected band sizing 524 bp, a larger band was also observed. Cloning and sequence analysis of the larger cDNA from two SCLC cell lines revealed a size of 643 bp due to the presence of additional 119 bp within the previously reported IL-15 cDNA sequence. The 119 bp sequence matched with an IL-15 genomic sequence downstream the IL-15 second coding exon and may represent a previously unreported alternative exon (exon A). The SCLC-associated IL-15 mRNA isoform has a shorter open reading frame (ORF) due to stop codons in exon A, followed by a new AUG codon. The predicted IL-15 precursor protein displays a shorter signal peptide but shares the same aminoacidic composition of mature IL-15 protein. A possible functional role of IL-15, different from 'IL-2-like' activity, in human tumours, is suggested.
KW - Alternative splicing
KW - Human IL-15
KW - RT-PCR
KW - Small cell lung cancer
UR - http://www.scopus.com/inward/record.url?scp=0030047144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030047144&partnerID=8YFLogxK
M3 - Article
C2 - 8668345
AN - SCOPUS:0030047144
VL - 12
SP - 2187
EP - 2192
JO - Oncogene
JF - Oncogene
SN - 0950-9232
IS - 10
ER -