Identification of a novel interleukin-15 (IL-15) transcript isoform generated by alternative splicing in human small cell lung cancer cell lines

Raffaella Meazza, Simonetta Verdiani, Roberto Biassoni, Massimiliano Coppolecchia, Alessia Gaggero, Anna Maria Orengo, Mario P. Colombo, Bruno Azzarone, Silvano Ferrini

Research output: Contribution to journalArticlepeer-review

Abstract

IL-15 is a cytokine promoting growth and differentiation of T, B and NK lymphocytes. By RT-PCR analysis, using primers allowing amplification of the entire IL-15 mRNA coding region, 9/11 small cell lung cancer (SCLC) cell lines displayed detectable IL-15 gene expression. In addition to the expected band sizing 524 bp, a larger band was also observed. Cloning and sequence analysis of the larger cDNA from two SCLC cell lines revealed a size of 643 bp due to the presence of additional 119 bp within the previously reported IL-15 cDNA sequence. The 119 bp sequence matched with an IL-15 genomic sequence downstream the IL-15 second coding exon and may represent a previously unreported alternative exon (exon A). The SCLC-associated IL-15 mRNA isoform has a shorter open reading frame (ORF) due to stop codons in exon A, followed by a new AUG codon. The predicted IL-15 precursor protein displays a shorter signal peptide but shares the same aminoacidic composition of mature IL-15 protein. A possible functional role of IL-15, different from 'IL-2-like' activity, in human tumours, is suggested.

Original languageEnglish
Pages (from-to)2187-2192
Number of pages6
JournalOncogene
Volume12
Issue number10
Publication statusPublished - 1996

Keywords

  • Alternative splicing
  • Human IL-15
  • RT-PCR
  • Small cell lung cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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