Identification of a novel mouse Dbl proto-oncogene splice variant: Evidence that SEC14 domain is involved in GEF activity regulation

Marzia Ognibene, Cristina Vanni, Fabiola Blengio, Daniela Segalerba, Patrizia Mancini, Patrizia De Marco, Maria R. Torrisi, Maria C. Bosco, Luigi Varesio, Alessandra Eva

Research output: Contribution to journalArticle

Abstract

The Rho guanine nucleotide exchange factor protoDbl is involved in different biochemical pathways affecting cell proliferation and migration. The N-terminal sequence of protoDbl contains negative regulatory elements that restrict the catalytic activity of the DH-PH module. Here, we report the identification of a new mouse protoDbl splice variant lacking exon 3. We found that the splice variant mRNA is expressed in the spleen and bone marrow lymphocytes, adrenal gland, gonads and brain. The protoDbl variant protein was detectable in the brain. The newly identified variant displays the disruption of the SEC14 domain, positioned on exons 2 and 3 in the protoDbl N-terminal region. We show here that an altered SEC14 sequence leads to enhanced Dbl translocation to the plasma membrane and to augmented transforming and exchange activity.

Original languageEnglish
Pages (from-to)220-229
Number of pages10
JournalGene
Volume537
Issue number2
DOIs
Publication statusPublished - Mar 10 2014

Keywords

  • Alternative splicing
  • ProtoDbl oncogene
  • Rho GTPases
  • SEC14 protein domain
  • Transforming activity

ASJC Scopus subject areas

  • Genetics

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