Identification of a novel type 1 diabetes-specific epitope by screening phage libraries with sera from pre-diabetic patients

Carmela Mennuni, Claudia Santini, Domenico Lazzaro, Francesco Dotta, Loredana Farilla, Alessandra Fierabracci, Gian Franco Bottazzo, Umberto Di Mario, Riccardo Cortese, Alessandra Luzzago

Research output: Contribution to journalArticle

Abstract

We used random peptide libraries displayed on phage to search for ligands to insulin dependent diabetes mellitus-related antibodies and were able to identify several candidate disease-related peptides. One of them, clone 92, showed a significant difference in the frequency of reactivity with the sera of patients and normal controls. Human immunoglobulins immunopurified on phage 92 specifically stained the islets on human pancreatic sections. When injected into rabbits, the selected peptide elicited antibodies that also stained human and rat pancreatic sections, with a pattern similar to that observed with immunoglobulins purified from the sera of patients. No reactivity was observed in other tissues. Our results indicate that the peptide identified in this work mimics a novel, diabetes-related self-antigen.

Original languageEnglish
Pages (from-to)599-606
Number of pages8
JournalJournal of Molecular Biology
Volume268
Issue number3
DOIs
Publication statusPublished - May 9 1997

Keywords

  • Autoantigens
  • Insulin dependent diabetes mellitus
  • Phage peptide libraries

ASJC Scopus subject areas

  • Virology

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    Mennuni, C., Santini, C., Lazzaro, D., Dotta, F., Farilla, L., Fierabracci, A., Bottazzo, G. F., Di Mario, U., Cortese, R., & Luzzago, A. (1997). Identification of a novel type 1 diabetes-specific epitope by screening phage libraries with sera from pre-diabetic patients. Journal of Molecular Biology, 268(3), 599-606. https://doi.org/10.1006/jmbi.1997.0946