Identification of a promiscuous T-cell epitope in Mycobacterium tuberculosis Mce proteins

M. Panigada, T. Sturniolo, G. Besozzi, M. G. Boccieri, F. Sinigaglia, G. G. Grassi, F. Grassi

Research output: Contribution to journalArticlepeer-review

Abstract

The characterization of Mycobacterium tuberculosis antigens inducing CD4+ T-cell responses could critically contribute to the development of subunit vaccines for M. tuberculosis. Here we performed computational analysis by using T-cell epitope prediction software (known as TEPITOPE) to predict promiscuous HLA-DR ligands in the products of the mce genes of M. tuberculosis. The analysis of the proliferative responses of CD4+ T cells from patients with pulmonary tuberculosis to selected peptides displaying promiscuous binding to HLA-DR in vitro led us to the identification of a peptide that induced proliferation of CD4+ cells from 50% of the tested subjects. This study demonstrates that a systematic computational approach can be used to identify T-cell epitopes in proteins expressed by an intracellular pathogen.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalInfection and Immunity
Volume70
Issue number1
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Immunology

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