Identification of a T cell gene expression clock obtained by exploiting a MZ twin design

Daniel Remondini, Nathan Intrator, Claudia Sala, Michela Pierini, Paolo Garagnani, Isabella Zironi, Claudio Franceschi, Stefano Salvioli, Gastone Castellani

Research output: Contribution to journalArticle

Abstract

Many studies investigated age-related changes in gene expression of different tissues, with scarce agreement due to the high number of affecting factors. Similarly, no consensus has been reached on which genes change expression as a function of age and not because of environment. In this study we analysed gene expression of T lymphocytes from 27 healthy monozygotic twin couples, with ages ranging over whole adult lifespan (22 to 98 years). This unique experimental design allowed us to identify genes involved in normative aging, which expression changes independently from environmental factors. We obtained a transcriptomic signature with 125 genes, from which chronological age can be estimated. This signature has been tested in two datasets of same cell type hybridized over two different platforms, showing a significantly better performance compared to random signatures. Moreover, the same signature was applied on a dataset from a different cell type (human muscle). A lower performance was obtained, indicating the possibility that the signature is T cell-specific. As a whole our results suggest that this approach can be useful to identify age-modulated genes.

Original languageEnglish
Article number6005
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

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T-Lymphocytes
Gene Expression
Genes
Monozygotic Twins
Muscle Cells
Research Design
Datasets

ASJC Scopus subject areas

  • General

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Remondini, D., Intrator, N., Sala, C., Pierini, M., Garagnani, P., Zironi, I., ... Castellani, G. (2017). Identification of a T cell gene expression clock obtained by exploiting a MZ twin design. Scientific Reports, 7(1), [6005]. https://doi.org/10.1038/s41598-017-05694-2

Identification of a T cell gene expression clock obtained by exploiting a MZ twin design. / Remondini, Daniel; Intrator, Nathan; Sala, Claudia; Pierini, Michela; Garagnani, Paolo; Zironi, Isabella; Franceschi, Claudio; Salvioli, Stefano; Castellani, Gastone.

In: Scientific Reports, Vol. 7, No. 1, 6005, 01.12.2017.

Research output: Contribution to journalArticle

Remondini, D, Intrator, N, Sala, C, Pierini, M, Garagnani, P, Zironi, I, Franceschi, C, Salvioli, S & Castellani, G 2017, 'Identification of a T cell gene expression clock obtained by exploiting a MZ twin design', Scientific Reports, vol. 7, no. 1, 6005. https://doi.org/10.1038/s41598-017-05694-2
Remondini D, Intrator N, Sala C, Pierini M, Garagnani P, Zironi I et al. Identification of a T cell gene expression clock obtained by exploiting a MZ twin design. Scientific Reports. 2017 Dec 1;7(1). 6005. https://doi.org/10.1038/s41598-017-05694-2
Remondini, Daniel ; Intrator, Nathan ; Sala, Claudia ; Pierini, Michela ; Garagnani, Paolo ; Zironi, Isabella ; Franceschi, Claudio ; Salvioli, Stefano ; Castellani, Gastone. / Identification of a T cell gene expression clock obtained by exploiting a MZ twin design. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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