Identification of an acidic metabolite of N-nitrosodiethanolamine isolated from rat urine

L. Airoldi, M. Bonfanti, E. Benfenati, P. Tavecchia, R. Fanelli

Research output: Contribution to journalArticle

Abstract

N-Nitrosodiethanolamine metabolism was studied in order to clarify the mechanism(s) by which this compound elicits its carcinogenic effect. Samples of 24 h urine from rats given a single dose of N-nitrosodiethanolamine were collected. Gas chromatographic thermal energy analyses showed the presence of only one compound containing the nitroso moiety besides N-nitrosodiethanolamine. This compound was identified as the acidic derivative N-(2-hydroxyethyl)-N-carboxymethylnitrosamine by comparison with an authentic standard by means of gas chromatographic mass spectrometric analysis of the trimethylsilyl or pentafluorobenzyl esters. The amount of N-(2-hydroxyethyl)-N-carboxymethylnitrosamine excreted in the 24 h urine was about 6% of the N-nitrosodiethanolamine administered.

Original languageEnglish
Pages (from-to)334-337
Number of pages4
JournalBiomedical Mass Spectrometry
Volume10
Issue number5
Publication statusPublished - 1983

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Metabolites
Rats
Urine
Thermal energy
Gases
Metabolism
Esters
Nitroso Compounds
Derivatives
Hot Temperature
N-nitrosodiethanolamine
N-(2-hydroxyethyl)-N-carboxymethylnitrosamine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

Cite this

Identification of an acidic metabolite of N-nitrosodiethanolamine isolated from rat urine. / Airoldi, L.; Bonfanti, M.; Benfenati, E.; Tavecchia, P.; Fanelli, R.

In: Biomedical Mass Spectrometry, Vol. 10, No. 5, 1983, p. 334-337.

Research output: Contribution to journalArticle

Airoldi, L, Bonfanti, M, Benfenati, E, Tavecchia, P & Fanelli, R 1983, 'Identification of an acidic metabolite of N-nitrosodiethanolamine isolated from rat urine', Biomedical Mass Spectrometry, vol. 10, no. 5, pp. 334-337.
Airoldi, L. ; Bonfanti, M. ; Benfenati, E. ; Tavecchia, P. ; Fanelli, R. / Identification of an acidic metabolite of N-nitrosodiethanolamine isolated from rat urine. In: Biomedical Mass Spectrometry. 1983 ; Vol. 10, No. 5. pp. 334-337.
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