Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

M. Pieretti, T. Beccari, E. V. Cosmi, J. L. Stirling, A. Orlacchio

Research output: Contribution to journalArticlepeer-review

Abstract

A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalClinica Chimica Acta
Volume188
Issue number2
DOIs
Publication statusPublished - Apr 30 1990

Keywords

  • Chorionic villi
  • Isoenzymes
  • Lysosome
  • Tay-Sachs disease
  • β-N-Acetylhexosaminidase

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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