Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

M. Pieretti; T. Beccari; E. V. Cosmi; J. L. Stirling; A. Orlacchio

doi:10.1016/0009-8981(90)90155-L

Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

M. Pieretti, T. Beccari, E. V. Cosmi, J. L. Stirling, A. Orlacchio

Fondazione Santa Lucia

Research output: Contribution to journal › Article › peer-review

Abstract

A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Clinica Chimica Acta
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(90)90155-L
Publication status	Published - Apr 30 1990

Keywords

Chorionic villi
Isoenzymes
Lysosome
Tay-Sachs disease
β-N-Acetylhexosaminidase

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

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title = "Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi",

abstract = "A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.",

keywords = "Chorionic villi, Isoenzymes, Lysosome, Tay-Sachs disease, β-N-Acetylhexosaminidase",

author = "M. Pieretti and T. Beccari and Cosmi, {E. V.} and Stirling, {J. L.} and A. Orlacchio",

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AU - Pieretti, M.

AU - Beccari, T.

AU - Cosmi, E. V.

AU - Stirling, J. L.

AU - Orlacchio, A.

PY - 1990/4/30

Y1 - 1990/4/30

N2 - A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

AB - A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

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KW - Isoenzymes

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KW - Tay-Sachs disease

KW - β-N-Acetylhexosaminidase

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