TY - JOUR
T1 - Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi
AU - Pieretti, M.
AU - Beccari, T.
AU - Cosmi, E. V.
AU - Stirling, J. L.
AU - Orlacchio, A.
PY - 1990/4/30
Y1 - 1990/4/30
N2 - A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.
AB - A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.
KW - Chorionic villi
KW - Isoenzymes
KW - Lysosome
KW - Tay-Sachs disease
KW - β-N-Acetylhexosaminidase
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U2 - 10.1016/0009-8981(90)90155-L
DO - 10.1016/0009-8981(90)90155-L
M3 - Article
C2 - 2143122
AN - SCOPUS:0025356856
VL - 188
SP - 109
EP - 117
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 2
ER -