Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

M. Pieretti; T. Beccari; E. V. Cosmi; J. L. Stirling; A. Orlacchio

doi:10.1016/0009-8981(90)90155-L

Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi

M. Pieretti, T. Beccari, E. V. Cosmi, J. L. Stirling, A. Orlacchio

Fondazione Santa Lucia

Research output: Contribution to journal › Article

2 Citations (Scopus)

Abstract

A minor form of β-N-acetylhexosaminidase has been found in chorionic villi, in addition to the major forms A and B. This form does not hydrolyze the 4-methyl-umbelliferyl-2-acetamido-2-deoxy-β-D-glucopyranoside-6-sul phate substrate, is thermostable, has a higher mol mass (120 000) than A and B (100 000) and on analytical isoelectric focusing, it shows a microhetereogeneity with values ranging between 6.3 and 7.0. For these characteristics, it resembles β-N-Acetylhexosaminidase P from pregnancy serum, from which is chromatographically indistinguishable.

Original language	English
Pages (from-to)	109-117
Number of pages	9
Journal	Clinica Chimica Acta
Volume	188
Issue number	2
DOIs	https://doi.org/10.1016/0009-8981(90)90155-L
Publication status	Published - Apr 30 1990

Fingerprint

Chorionic Villi

Isoelectric Focusing

Pregnancy

Substrates

Serum

methyl-2-acetamido-2-deoxy-D-glucopyranoside

Keywords

Chorionic villi
Isoenzymes
Lysosome
Tay-Sachs disease
β-N-Acetylhexosaminidase

ASJC Scopus subject areas

Biochemistry
Clinical Biochemistry

Cite this

Pieretti, M., Beccari, T., Cosmi, E. V., Stirling, J. L., & Orlacchio, A. (1990). Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi. Clinica Chimica Acta, 188(2), 109-117. https://doi.org/10.1016/0009-8981(90)90155-L

Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi. / Pieretti, M.; Beccari, T.; Cosmi, E. V.; Stirling, J. L.; Orlacchio, A.

In: Clinica Chimica Acta, Vol. 188, No. 2, 30.04.1990, p. 109-117.

Research output: Contribution to journal › Article

Pieretti, M, Beccari, T, Cosmi, EV, Stirling, JL & Orlacchio, A 1990, 'Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi', Clinica Chimica Acta, vol. 188, no. 2, pp. 109-117. https://doi.org/10.1016/0009-8981(90)90155-L

Pieretti M, Beccari T, Cosmi EV, Stirling JL, Orlacchio A. Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi. Clinica Chimica Acta. 1990 Apr 30;188(2):109-117. https://doi.org/10.1016/0009-8981(90)90155-L

Pieretti, M. ; Beccari, T. ; Cosmi, E. V. ; Stirling, J. L. ; Orlacchio, A. / Identification of an intermediate form of β-N-acetylhexosaminidase in chorionic villi. In: Clinica Chimica Acta. 1990 ; Vol. 188, No. 2. pp. 109-117.

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10.1016/0009-8981(90)90155-L