Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells

Irene Colavita, Nicola Esposito, Concetta Quintarelli, Ersilia Nigro, Fabrizio Pane, Margherita Ruoppolo, Francesco Salvatore

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In the present study, we used a functional proteomic approach to identify Annexin A1 (Anxa1) interacting proteins in the Philadelphia-positive KCL22 cell line. We focused on Anxa1 because it is one of the major proteins upregulated in imatinib-sensitive KCL22S cells versus imatinib-resistant KCL22R. Our proteomic strategy revealed 21 interactors. Bioinformatic analysis showed that most of these proteins are involved in cell death processes. Among the proteins identified, we studied the interaction of Anxa1 with two phosphatases, Shp1 and Shp2, which were recently identified as biomarkers of imatinib sensitivity in patients affected by chronic myeloid leukemia. Our data open new perspectives in the search for annexin-mediated signaling pathways and may shed light on mechanisms of resistance to imatinib that are unrelated to Bcr-Abl activity. All mass spectrometry data have been deposited in the ProteomeXchange with identifier PXD000030.

Original languageEnglish
Pages (from-to)2414-2418
Number of pages5
JournalProteomics
Volume13
Issue number16
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Annexins
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Annexin A1
Proteomics
Proteins
Biomarkers
Cell death
Bioinformatics
Computational Biology
Phosphoric Monoester Hydrolases
Mass spectrometry
Mass Spectrometry
Cell Death
Cells
Cell Line
Imatinib Mesylate

Keywords

  • Annexin A1
  • Chronic myeloid leukemia
  • Imatinib resistance
  • Protein interaction

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Colavita, I., Esposito, N., Quintarelli, C., Nigro, E., Pane, F., Ruoppolo, M., & Salvatore, F. (2013). Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells. Proteomics, 13(16), 2414-2418. https://doi.org/10.1002/pmic.201200444

Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells. / Colavita, Irene; Esposito, Nicola; Quintarelli, Concetta; Nigro, Ersilia; Pane, Fabrizio; Ruoppolo, Margherita; Salvatore, Francesco.

In: Proteomics, Vol. 13, No. 16, 08.2013, p. 2414-2418.

Research output: Contribution to journalArticle

Colavita, I, Esposito, N, Quintarelli, C, Nigro, E, Pane, F, Ruoppolo, M & Salvatore, F 2013, 'Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells', Proteomics, vol. 13, no. 16, pp. 2414-2418. https://doi.org/10.1002/pmic.201200444
Colavita, Irene ; Esposito, Nicola ; Quintarelli, Concetta ; Nigro, Ersilia ; Pane, Fabrizio ; Ruoppolo, Margherita ; Salvatore, Francesco. / Identification of Annexin A1 interacting proteins in chronic myeloid leukemia KCL22 cells. In: Proteomics. 2013 ; Vol. 13, No. 16. pp. 2414-2418.
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