Identification of Apolipoproteins A-I and B Using High Resolution Electrophoresis on Supported Cellulose Acetate

Soluzzo Cavalcanti, Ernesta Cavalcanti, Elvira Angotti, Alessandra Liparoti, Alfredo Colonna

Research output: Contribution to journalArticle

Abstract

A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on —20 °C stored serum samples.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume33
Issue number5
DOIs
Publication statusPublished - 1995

Fingerprint

Apolipoprotein A-I
Apolipoproteins B
Electrophoresis
Polysorbates
Apolipoproteins
Surface-Active Agents
Blood Proteins
Buffers
Serum
acetylcellulose

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Identification of Apolipoproteins A-I and B Using High Resolution Electrophoresis on Supported Cellulose Acetate. / Cavalcanti, Soluzzo; Cavalcanti, Ernesta; Angotti, Elvira; Liparoti, Alessandra; Colonna, Alfredo.

In: Clinical Chemistry and Laboratory Medicine, Vol. 33, No. 5, 1995, p. 275-280.

Research output: Contribution to journalArticle

Cavalcanti, Soluzzo ; Cavalcanti, Ernesta ; Angotti, Elvira ; Liparoti, Alessandra ; Colonna, Alfredo. / Identification of Apolipoproteins A-I and B Using High Resolution Electrophoresis on Supported Cellulose Acetate. In: Clinical Chemistry and Laboratory Medicine. 1995 ; Vol. 33, No. 5. pp. 275-280.
@article{7a07dc374a084800aefead6ad89620aa,
title = "Identification of Apolipoproteins A-I and B Using High Resolution Electrophoresis on Supported Cellulose Acetate",
abstract = "A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on —20 °C stored serum samples.",
author = "Soluzzo Cavalcanti and Ernesta Cavalcanti and Elvira Angotti and Alessandra Liparoti and Alfredo Colonna",
year = "1995",
doi = "10.1515/cclm.1995.33.5.275",
language = "English",
volume = "33",
pages = "275--280",
journal = "Clinical Chemistry and Laboratory Medicine",
issn = "1434-6621",
publisher = "Walter de Gruyter GmbH",
number = "5",

}

TY - JOUR

T1 - Identification of Apolipoproteins A-I and B Using High Resolution Electrophoresis on Supported Cellulose Acetate

AU - Cavalcanti, Soluzzo

AU - Cavalcanti, Ernesta

AU - Angotti, Elvira

AU - Liparoti, Alessandra

AU - Colonna, Alfredo

PY - 1995

Y1 - 1995

N2 - A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on —20 °C stored serum samples.

AB - A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on —20 °C stored serum samples.

UR - http://www.scopus.com/inward/record.url?scp=84943472900&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943472900&partnerID=8YFLogxK

U2 - 10.1515/cclm.1995.33.5.275

DO - 10.1515/cclm.1995.33.5.275

M3 - Article

AN - SCOPUS:84943472900

VL - 33

SP - 275

EP - 280

JO - Clinical Chemistry and Laboratory Medicine

JF - Clinical Chemistry and Laboratory Medicine

SN - 1434-6621

IS - 5

ER -