Identification of apolipoproteins A-I and B using high resolution electrophoresis on supported cellulose acetate

S. Cavalcanti, E. Cavalcanti, E. Angotti, A. Liparoti, A. Colonna

Research output: Contribution to journalArticle

Abstract

A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on -20°C stored serum samples.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalEuropean Journal of Clinical Chemistry and Clinical Biochemistry
Volume33
Issue number5
Publication statusPublished - 1995

Fingerprint

Apolipoprotein A-I
Apolipoproteins B
Electrophoresis
Polysorbates
Apolipoproteins
Surface-Active Agents
Blood Proteins
Buffers
Serum
acetylcellulose

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Identification of apolipoproteins A-I and B using high resolution electrophoresis on supported cellulose acetate. / Cavalcanti, S.; Cavalcanti, E.; Angotti, E.; Liparoti, A.; Colonna, A.

In: European Journal of Clinical Chemistry and Clinical Biochemistry, Vol. 33, No. 5, 1995, p. 275-279.

Research output: Contribution to journalArticle

@article{81933e64077242deb2695a50316057b6,
title = "Identification of apolipoproteins A-I and B using high resolution electrophoresis on supported cellulose acetate",
abstract = "A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on -20°C stored serum samples.",
author = "S. Cavalcanti and E. Cavalcanti and E. Angotti and A. Liparoti and A. Colonna",
year = "1995",
language = "English",
volume = "33",
pages = "275--279",
journal = "European Journal of Clinical Chemistry and Clinical Biochemistry",
issn = "0939-4974",
publisher = "Walter de Gruyter GmbH & Co. KG",
number = "5",

}

TY - JOUR

T1 - Identification of apolipoproteins A-I and B using high resolution electrophoresis on supported cellulose acetate

AU - Cavalcanti, S.

AU - Cavalcanti, E.

AU - Angotti, E.

AU - Liparoti, A.

AU - Colonna, A.

PY - 1995

Y1 - 1995

N2 - A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on -20°C stored serum samples.

AB - A simple modification of high resolution electrophoresis on supported cellulose acetate is described. This modification is made by adding the surfactant Tween 20 to the buffer and permits the identification of apolipoproteins A-I and B as well as the usually detected serum proteins. The procedure is reproducible using various supported cellulose acetate plates with manual and automated procedures. Furthermore, this improved high resolution electrophoresis allows the semiquantitative evaluation of these apolipoproteins both on freshly collected and on -20°C stored serum samples.

UR - http://www.scopus.com/inward/record.url?scp=0029001580&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029001580&partnerID=8YFLogxK

M3 - Article

VL - 33

SP - 275

EP - 279

JO - European Journal of Clinical Chemistry and Clinical Biochemistry

JF - European Journal of Clinical Chemistry and Clinical Biochemistry

SN - 0939-4974

IS - 5

ER -