TY - JOUR
T1 - Identification of CCR8
T2 - A human monocyte and thymus receptor for the CC chemokine I-309
AU - Tiffany, H. Lee
AU - Lautens, Laura L.
AU - Gao, Ji Liang
AU - Pease, James
AU - Locati, Massimo
AU - Combadiere, Christophe
AU - Modi, William
AU - Bonner, Tom I.
AU - Murphy, Philip M.
PY - 1997/7/7
Y1 - 1997/7/7
N2 - The human CC chemokine I-309 is a potent monocyte chemoattractant and inhibits apoptosis in thymic cell lines. Here, we identify a specific human I-309 receptor, and name it CCR8 according to an accepted nomenclature system. The receptor has seven predicted transmembrane domains, is expressed constitutively in monocytes and thymus, and is encoded by a previously reported gent of previously unknown function named, alternatively, CY6, TER1, and CKR-L1. After transfection with the CY6 open reading frame, a mouse pre- B cell line exhibited calcium flux and chemotaxis in response to 1-309 (EC50 = 2 nM for each), whereas 20 other chemokines were inactive. Signaling was sensitive to pertussis toxin, suggesting coupling to a G(i)- type G protein. These properties parallel those of endogenous I-309 receptors expressed in an HL-60 clone 15 cell line model. The apparent monogamous relationship between I-309 and CCR8 is unusual among known CC chemokines and known CC chemokine receptors. CCR8 may regulate monocyte chemotaxis and thymic cell line apoptosis.
AB - The human CC chemokine I-309 is a potent monocyte chemoattractant and inhibits apoptosis in thymic cell lines. Here, we identify a specific human I-309 receptor, and name it CCR8 according to an accepted nomenclature system. The receptor has seven predicted transmembrane domains, is expressed constitutively in monocytes and thymus, and is encoded by a previously reported gent of previously unknown function named, alternatively, CY6, TER1, and CKR-L1. After transfection with the CY6 open reading frame, a mouse pre- B cell line exhibited calcium flux and chemotaxis in response to 1-309 (EC50 = 2 nM for each), whereas 20 other chemokines were inactive. Signaling was sensitive to pertussis toxin, suggesting coupling to a G(i)- type G protein. These properties parallel those of endogenous I-309 receptors expressed in an HL-60 clone 15 cell line model. The apparent monogamous relationship between I-309 and CCR8 is unusual among known CC chemokines and known CC chemokine receptors. CCR8 may regulate monocyte chemotaxis and thymic cell line apoptosis.
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U2 - 10.1084/jem.186.1.165
DO - 10.1084/jem.186.1.165
M3 - Article
C2 - 9207005
AN - SCOPUS:0030845591
VL - 186
SP - 165
EP - 170
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 1
ER -