Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8

Fabiola Micheletti, Paolo Monini, Cinzia Fortini, Paola Rimessi, Martina Bazzaro, Massimo Andreoni, Massimo Giuliani, Serena Traniello, Barbara Ensoli, Riccardo Gavioli

Research output: Contribution to journalArticle

Abstract

The human herpesvirus 8 (HHV-8) is a human γ2-herpesvirus that is implicated in the development of Kaposi's sarcoma (KS), primary effusion lymphoma and Castelman's disease. Since the responses of cytotoxic T lymphocytes (CTL) play a key role in the control of herpesvirus infection, it is important to identify and to characterize the CTL target epitopes of HHV-8 viral antigens. In this study, using peptide-binding motifs, we selected potential human leucocyte antigen (HLA)-A2-binding peptides from kaposin A and glycoprotein H (gH), that are latent and lytic HHV-8 antigens, respectively. HLA-A2-binding peptides were tested for their capacity to induce CTL responses in HHV-8-negative healthy donors. By this approach, we found that the majority of individuals responded to two HHV-8-derived CTL epitopes, namely, VLLNGWRWRL (amino acids 16-25), which derives from kaposin A, and FLNWQNLLNV (amino acids 59-68), which derives from gH. In addition, memory CTL responses to these epitopes were detected in disease-free individuals infected by HHV-8 demonstrating that the two epitopes are relevant targets of CTL-mediated immunity in vivo. The identified epitopes may be investigated for the development of immunotherapeutic strategies against HHV-8-associated malignancies.

Original languageEnglish
Pages (from-to)395-403
Number of pages9
JournalImmunology
Volume106
Issue number3
DOIs
Publication statusPublished - 2002

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Human Herpesvirus 8
T-Lymphocyte Epitopes
Cytotoxic T-Lymphocytes
Epitopes
HLA Antigens
Glycoproteins
Primary Effusion Lymphoma
Amino Acids
Herpesviridae Infections
Human Herpesvirus 2
Viral Antigens
Kaposi's Sarcoma
Immunity
Antigens
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

Micheletti, F., Monini, P., Fortini, C., Rimessi, P., Bazzaro, M., Andreoni, M., ... Gavioli, R. (2002). Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8. Immunology, 106(3), 395-403. https://doi.org/10.1046/j.1365-2567.2002.01424.x

Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8. / Micheletti, Fabiola; Monini, Paolo; Fortini, Cinzia; Rimessi, Paola; Bazzaro, Martina; Andreoni, Massimo; Giuliani, Massimo; Traniello, Serena; Ensoli, Barbara; Gavioli, Riccardo.

In: Immunology, Vol. 106, No. 3, 2002, p. 395-403.

Research output: Contribution to journalArticle

Micheletti, F, Monini, P, Fortini, C, Rimessi, P, Bazzaro, M, Andreoni, M, Giuliani, M, Traniello, S, Ensoli, B & Gavioli, R 2002, 'Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8', Immunology, vol. 106, no. 3, pp. 395-403. https://doi.org/10.1046/j.1365-2567.2002.01424.x
Micheletti F, Monini P, Fortini C, Rimessi P, Bazzaro M, Andreoni M et al. Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8. Immunology. 2002;106(3):395-403. https://doi.org/10.1046/j.1365-2567.2002.01424.x
Micheletti, Fabiola ; Monini, Paolo ; Fortini, Cinzia ; Rimessi, Paola ; Bazzaro, Martina ; Andreoni, Massimo ; Giuliani, Massimo ; Traniello, Serena ; Ensoli, Barbara ; Gavioli, Riccardo. / Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8. In: Immunology. 2002 ; Vol. 106, No. 3. pp. 395-403.
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