Identification of differentially expressed genes in coronary atherosclerotic plaques from patients with stable or unstable angina by cDNA array analysis

A. M. Randi, E. Biguzzi, F. Falciani, P. Merlini, S. Blakemore, E. Bramucci, S. Lucreziotti, M. Lennon, E. M. Faioni, D. Ardi SsiNo, P. M. Mannucci

Research output: Contribution to journalArticlepeer-review

Abstract

The composition of atherosclerotic plaques is a crucial factor in determining rupture, thrombosis and clinical events. In this study, we analyzed gene expression in coronary plaques from patients with stable or unstable angina using gene arrays. Total RNA was extracted from eight plaques collected by therapeutic directional coronary atherectomy. cDNA probes, generated by amplification, were hybridized to nylon arrays containing 482 genes. Here we report the results for the inflammation, adhesion and hemostasis subsets. Many genes not previously associated with atherosclerosis, such as the lymphocyte adhesion molecule MadCAM, were expressed in the plaques. ANOVA analysis showed higher tissue factor (TF) expression in unstable angina samples. Five genes were expressed at lower levels in unstable angina samples: anticoagulant protein S, cyclooxygenase (COX)-l, interleukin (IL)-7 and chemokines monocyte chemotactic protein (MCP)-l and -2. Gene arrays provide a new approach to study plaque composition and identify candidate markers of plaque instability.

Original languageEnglish
Pages (from-to)829-835
Number of pages7
JournalJournal of Thrombosis and Haemostasis
Volume1
Issue number4
DOIs
Publication statusPublished - Apr 2003

Keywords

  • Angina
  • Atherosclerosis
  • Gene expression
  • Molecular biology

ASJC Scopus subject areas

  • Hematology
  • Medicine(all)

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