Identification of domains responsible for von Willebrand factor type VI collagen interaction mediating platelet adhesion under high flow

Mario Mazzucato, Paola Spessotto, Adriana Masotti, Leandro De Appollonia, Maria Rita Cozzi, Akira Yoshioka, Roberto Perris, Alfonso Colombatti, Luigi De Marco

Research output: Contribution to journalArticle

Abstract

We have identified type VI collagen (Col VI) as a primary subendothelial extracellular matrix component responsible for yon Willebrand factor (vWF)- dependent platelet adhesion and aggregation under high tensile strength. Intact tetrameric Col VI was the form of the collagen found to be capable of promoting vWF-mediated platelet adhesion/aggregation under this shear condition, whereas removal of the predominant portion of the terminal globules by pepsin treatment abrogated its activity. The inability of the pepsin-digested Col VI to support any platelet interaction at high flow was because of the failure of the A3(vWF) domain to bind to this form of collagen, suggesting a stringent requirement of a tridimensional conformation or of intactness of its macromolecular structure. In contrast, the A1(vWF) domain bound to both intact and pepsin-digested Col VI tetramers but, in accordance with the cooperating function of the two vWF domains, failed to support platelet adhesion/aggregation under high shear onto Col VI by itself. The putative A1(vWF) binding site resided within the A7(VI) module (residues 413-613) of the globular amino-terminal portion of the α3(VI) chain. Soluble recombinant A7(VI) polypeptide strongly perturbed the vWF-mediated platelet adhesion to Col VI under high shear rates, without affecting the binding of the vWF platelet receptor glycoprotein Ibα to its cognate ligand A1(vWF). The findings provide evidence for a concerted action of the A1(vWF) and A3(vWF) domains in inducing platelet arrest on Col VI. This is accomplished via an interaction of the A1(vWF) domain with a site contained in the α3 chain A7(VI) domain and via a conformation-dependent interaction of the A3(vWF) domain with the intact tetrameric collagen. The data further emphasize that Col VI microfilaments linking the subendothelial basement membrane to the interstitial collagenous network may play a pivotal role in the hemostatic process triggered upon damage of the blood vessel wall.

Original languageEnglish
Pages (from-to)3033-3041
Number of pages9
JournalJournal of Biological Chemistry
Volume274
Issue number5
DOIs
Publication statusPublished - Jan 29 1999

ASJC Scopus subject areas

  • Biochemistry

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