Identification of extracellular vesicles and characterization of miRNA expression profiles in human blastocoel fluid

R. Battaglia, S. Palini, M. E. Vento, A. La Ferlita, M. J. Lo Faro, E. Caroppo, P. Borzì, L. Falzone, D. Barbagallo, M. Ragusa, M. Scalia, G. D'Amato, P. Scollo, P. Musumeci, M. Purrello, E. Gravotta, C. Di Pietro

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, for the first time, we demonstrated the presence of microRNAs and extracellular vesicles in human blastocoel fluid. The bioinformatic and comparative analyses identified the biological function of blastocoel fluid microRNAs and suggested a potential role inside the human blastocyst. We found 89 microRNAs, expressed at different levels, able to regulate critical signaling pathways controlling embryo development, such as pluripotency, cell reprogramming, epigenetic modifications, intercellular communication, cell adhesion and cell fate. Blastocoel fluid microRNAs reflect the miRNome of embryonic cells and their presence, associated with the discovery of extracellular vesicles, inside blastocoel fluid, strongly suggests their important role in mediating cell communication among blastocyst cells. Their characterization is important to better understand the earliest stages of embryogenesis and the complex circuits regulating pluripotency. Moreover, blastocoel fluid microRNA profiles could be influenced by blastocyst quality, therefore, microRNAs might be used to assess embryo potential in IVF cycles.

Original languageEnglish
Number of pages1
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 14 2019

ASJC Scopus subject areas

  • General

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