Identification of GALNT14 as a novel neuroblastoma predisposition gene

Marilena De Mariano, Roberta Gallesio, Marco Chierici, Cesare Furlanello, Massimo Conte, Alberto Garaventa, Michela Croce, Silvano Ferrini, Gian Paolo Tonini, Luca Longo

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Although several genes have been associated to neuroblastoma (NB) predisposition and aggressiveness, further genes are likely involved in the overall risk of developing this pediatric cancer. We thus carried out whole-exome sequencing on germline DNA from two affected second cousins and two unlinked healthy relatives from a large family with hereditary NB. Bioinformatics analysis revealed 6999 variations that were exclusively shared by the two familial NB cases. We then considered for further analysis all unknown or rare missense mutations, which involved 30 genes. Validation and analysis of these variants led to identify a GALNT14 mutation (c.802C > T) that properly segregated in the family and was predicted as functionally damaging by PolyPhen2 and SIFT. Screening of 8 additional NB families and 167 sporadic cases revealed this GALNT14 mutation in the tumors of two twins and in the germline of one sporadic NB patient. Moreover, a significant association between MYCN amplification and GALNT14 expression was observed in both NB patients and cell lines. Also, GALNT14 higher expression is associated with a worse OS in a public dataset of 88 NB samples (http://r2.amc.nl). GALNT14 is a member of the polypeptide N-acetylgalactosaminyl-transferase family and maps closely to ALK on 2p23.1, a region we previously discovered in linkage with NB in the family here considered. The aberrant function of GALNTs can result in altered glycoproteins that have been associated to the promotion of tumor aggressiveness in various cancers. Although rare, the recurrence of this mutation suggests GALNT14 as a novel gene potentially involved in NB predisposition.

Original languageEnglish
Pages (from-to)26335-26346
Number of pages12
JournalOncotarget
Volume6
Issue number28
DOIs
Publication statusPublished - 2015

Fingerprint

Neuroblastoma
Genes
Mutation
Neoplasms
Exome
Missense Mutation
Transferases
Computational Biology
Glycoproteins
Pediatrics
Recurrence
Cell Line
Peptides
DNA

Keywords

  • Exome-sequencing
  • GALNT14
  • Genetic predisposition
  • Neuroblastoma
  • O-glycosylation

ASJC Scopus subject areas

  • Oncology

Cite this

Mariano, M. D., Gallesio, R., Chierici, M., Furlanello, C., Conte, M., Garaventa, A., ... Longo, L. (2015). Identification of GALNT14 as a novel neuroblastoma predisposition gene. Oncotarget, 6(28), 26335-26346. https://doi.org/10.18632/oncotarget.4501

Identification of GALNT14 as a novel neuroblastoma predisposition gene. / Mariano, Marilena De; Gallesio, Roberta; Chierici, Marco; Furlanello, Cesare; Conte, Massimo; Garaventa, Alberto; Croce, Michela; Ferrini, Silvano; Tonini, Gian Paolo; Longo, Luca.

In: Oncotarget, Vol. 6, No. 28, 2015, p. 26335-26346.

Research output: Contribution to journalArticle

Mariano, MD, Gallesio, R, Chierici, M, Furlanello, C, Conte, M, Garaventa, A, Croce, M, Ferrini, S, Tonini, GP & Longo, L 2015, 'Identification of GALNT14 as a novel neuroblastoma predisposition gene', Oncotarget, vol. 6, no. 28, pp. 26335-26346. https://doi.org/10.18632/oncotarget.4501
Mariano, Marilena De ; Gallesio, Roberta ; Chierici, Marco ; Furlanello, Cesare ; Conte, Massimo ; Garaventa, Alberto ; Croce, Michela ; Ferrini, Silvano ; Tonini, Gian Paolo ; Longo, Luca. / Identification of GALNT14 as a novel neuroblastoma predisposition gene. In: Oncotarget. 2015 ; Vol. 6, No. 28. pp. 26335-26346.
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