TY - JOUR
T1 - Identification of genes down-regulated during lung cancer progression
T2 - A cDNA array study
AU - Campioni, Mara
AU - Ambrogi, Vincenzo
AU - Pompeo, Eugenio
AU - Citro, Gennaro
AU - Castelli, Mauro
AU - Spugnini, Enrico P.
AU - Gatti, Antonio
AU - Cardelli, Pierluigi
AU - Lorenzon, Laura
AU - Baldi, Alfonso
AU - Mineo, Tommaso C.
PY - 2008
Y1 - 2008
N2 - Background. Lung cancer remains a major health challenge in the world. Survival for patients with stage I disease ranges between 40-70%. This suggests that a significant proportion of patients with stage I NSCLC may actually be under-staged. Methods. In order to identify genes relevant for lung cancer development, we carried out cDNA array experiments employing 64 consecutive patients (58 men and 6 women) with a median age of 58 years and stage 1 or stage 2 non-small-cell lung cancer (NSCLC). Results. Basic cDNA array data identified 14 genes as differentially regulated in the two groups. Quantitative RT-PCR analysis confirmed an effective different transcriptional regulation of 8 out of 14 genes analyzed. The products of these genes belong to different functional protein types, such as extra-cellular matrix proteins and proteases (Decorin and MMP11), genes involved in DNA repair (XRCC1), regulator of angiogenesis (VEGF), cell cycle regulators (Cyclin D1) and tumor-suppressor genes (Semaphorin 3B, WNT-5A and retinoblastoma-related Rb2/p130). Some previously described differences in expression patterns were confirmed by our array data. In addition, we identified and validated for the first time the reduced expression level of some genes during lung cancer progression. Conclusion. Comparative hybridization by means of cDNA arrays assisted in identifying a series of novel progression-associated changes in gene expression, confirming, at the same time, a number of previously described results.
AB - Background. Lung cancer remains a major health challenge in the world. Survival for patients with stage I disease ranges between 40-70%. This suggests that a significant proportion of patients with stage I NSCLC may actually be under-staged. Methods. In order to identify genes relevant for lung cancer development, we carried out cDNA array experiments employing 64 consecutive patients (58 men and 6 women) with a median age of 58 years and stage 1 or stage 2 non-small-cell lung cancer (NSCLC). Results. Basic cDNA array data identified 14 genes as differentially regulated in the two groups. Quantitative RT-PCR analysis confirmed an effective different transcriptional regulation of 8 out of 14 genes analyzed. The products of these genes belong to different functional protein types, such as extra-cellular matrix proteins and proteases (Decorin and MMP11), genes involved in DNA repair (XRCC1), regulator of angiogenesis (VEGF), cell cycle regulators (Cyclin D1) and tumor-suppressor genes (Semaphorin 3B, WNT-5A and retinoblastoma-related Rb2/p130). Some previously described differences in expression patterns were confirmed by our array data. In addition, we identified and validated for the first time the reduced expression level of some genes during lung cancer progression. Conclusion. Comparative hybridization by means of cDNA arrays assisted in identifying a series of novel progression-associated changes in gene expression, confirming, at the same time, a number of previously described results.
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U2 - 10.1186/1756-9966-27-38
DO - 10.1186/1756-9966-27-38
M3 - Article
C2 - 18793406
AN - SCOPUS:52949090360
VL - 27
JO - Journal of Experimental and Clinical Cancer Research
JF - Journal of Experimental and Clinical Cancer Research
SN - 0392-9078
IS - 1
M1 - 38
ER -