Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis

Mirella Sari-Gorla, Luca Rampoldi, Giorgio Binelli, Carla Frova, M. Enrico Pè

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.

Original languageEnglish
Pages (from-to)551-555
Number of pages5
JournalMGG Molecular & General Genetics
Volume251
Issue number5
DOIs
Publication statusPublished - 1996

Fingerprint

Pollen
Zea mays
Restriction Fragment Length Polymorphisms
Herbicide Resistance
Quantitative Trait Loci
Herbicides
Population
Regression Analysis
alachlor
Genes

Keywords

  • Gametophytic selection
  • Herbicide
  • Linkage analysis
  • Maize
  • Molecular markers

ASJC Scopus subject areas

  • Genetics

Cite this

Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis. / Sari-Gorla, Mirella; Rampoldi, Luca; Binelli, Giorgio; Frova, Carla; Pè, M. Enrico.

In: MGG Molecular & General Genetics, Vol. 251, No. 5, 1996, p. 551-555.

Research output: Contribution to journalArticle

Sari-Gorla, Mirella ; Rampoldi, Luca ; Binelli, Giorgio ; Frova, Carla ; Pè, M. Enrico. / Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis. In: MGG Molecular & General Genetics. 1996 ; Vol. 251, No. 5. pp. 551-555.
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