Abstract
The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.
| Original language | English |
|---|---|
| Pages (from-to) | 277-280 |
| Number of pages | 4 |
| Journal | Anticancer Research |
| Volume | 19 |
| Issue number | 1 A |
| Publication status | Published - 1999 |
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Keywords
- Differential display
- Genetic markers
- Glial tumor
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Identification of genetic markers of the glial tumor by means of Differential Display technique. / Comincini, Sergio; Capelli, Enrica; Cattana, Elena; Zanoli, Elisa; Ceroni, Mauro; Nano, Rosanna.
In: Anticancer Research, Vol. 19, No. 1 A, 1999, p. 277-280.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of genetic markers of the glial tumor by means of Differential Display technique
AU - Comincini, Sergio
AU - Capelli, Enrica
AU - Cattana, Elena
AU - Zanoli, Elisa
AU - Ceroni, Mauro
AU - Nano, Rosanna
PY - 1999
Y1 - 1999
N2 - The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.
AB - The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.
KW - Differential display
KW - Genetic markers
KW - Glial tumor
UR - http://www.scopus.com/inward/record.url?scp=0032897375&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032897375&partnerID=8YFLogxK
M3 - Article
C2 - 10226554
AN - SCOPUS:0032897375
VL - 19
SP - 277
EP - 280
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 1 A
ER -