Identification of genetic markers of the glial tumor by means of Differential Display technique

Sergio Comincini; Enrica Capelli; Elena Cattana; Elisa Zanoli; Mauro Ceroni; Rosanna Nano

Identification of genetic markers of the glial tumor by means of Differential Display technique

Sergio Comincini, Enrica Capelli, Elena Cattana, Elisa Zanoli, Mauro Ceroni, Rosanna Nano

Fondazione "Istituto Neurologico Casimiro Mondino"

Research output: Contribution to journal › Article › peer-review

Abstract

The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.

Original language	English
Pages (from-to)	277-280
Number of pages	4
Journal	Anticancer Research
Volume	19
Issue number	1 A
Publication status	Published - 1999

Keywords

Differential display
Genetic markers
Glial tumor

ASJC Scopus subject areas

Cancer Research
Oncology

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abstract = "The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.",

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T1 - Identification of genetic markers of the glial tumor by means of Differential Display technique

AU - Comincini, Sergio

AU - Capelli, Enrica

AU - Cattana, Elena

AU - Zanoli, Elisa

AU - Ceroni, Mauro

AU - Nano, Rosanna

PY - 1999

Y1 - 1999

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AB - The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.

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KW - Genetic markers

KW - Glial tumor

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