Identification of genetic markers of the glial tumor by means of Differential Display technique

Sergio Comincini, Enrica Capelli, Elena Cattana, Elisa Zanoli, Mauro Ceroni, Rosanna Nano

Research output: Contribution to journalArticle

Abstract

The Differential Display Reverse Transcriptase (DDRT) technique was adopted to isolate genetic markers specific for the two main grades of the Glial tumour, the Astrocytoma and the Glioblastoma. A total of 16 brain biopsies (4 Astrocytoma and 12 Glioblastoma) were analysed. The technique was modified in order to reduce the false-positive ratio by means of more stringent amplification conditions. Electrophoretic patterns with previously selected arbitarily primers revealed differences between the grades, four of them were investigated through sequencing. These sequences did not show significant nucleotide and aminoacid similarity to any known sequences in the DataBase. Sensitivity of the method was documented by the evidence that only one of the selected markers was an artefact, while others represented genetic markers of the human Glial neoplasm.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalAnticancer Research
Volume19
Issue number1 A
Publication statusPublished - 1999

Fingerprint

Astrocytoma
Glioblastoma
Genetic Markers
Neuroglia
RNA-Directed DNA Polymerase
Artifacts
Neoplasms
Nucleotides
Databases
Biopsy
Brain

Keywords

  • Differential display
  • Genetic markers
  • Glial tumor

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Comincini, S., Capelli, E., Cattana, E., Zanoli, E., Ceroni, M., & Nano, R. (1999). Identification of genetic markers of the glial tumor by means of Differential Display technique. Anticancer Research, 19(1 A), 277-280.

Identification of genetic markers of the glial tumor by means of Differential Display technique. / Comincini, Sergio; Capelli, Enrica; Cattana, Elena; Zanoli, Elisa; Ceroni, Mauro; Nano, Rosanna.

In: Anticancer Research, Vol. 19, No. 1 A, 1999, p. 277-280.

Research output: Contribution to journalArticle

Comincini, S, Capelli, E, Cattana, E, Zanoli, E, Ceroni, M & Nano, R 1999, 'Identification of genetic markers of the glial tumor by means of Differential Display technique', Anticancer Research, vol. 19, no. 1 A, pp. 277-280.
Comincini, Sergio ; Capelli, Enrica ; Cattana, Elena ; Zanoli, Elisa ; Ceroni, Mauro ; Nano, Rosanna. / Identification of genetic markers of the glial tumor by means of Differential Display technique. In: Anticancer Research. 1999 ; Vol. 19, No. 1 A. pp. 277-280.
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