Identification of grape and wine allergens as an endochitinase 4, a lipid-transfer protein, and a thaumatin

Elide Anna Pastorello, Laura Farioli, Valerio Pravettoni, Claudio Ortolani, Donatella Fortunato, Maria Gabriella Giuffrida, Lorenza Perono Garoffo, Ambra Marianna Calamari, Oreste Brenna, Amedeo Conti

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Abstract

Background: Few allergic reactions to grape are reported in the literature. In some cases an association with peach and cherry allergy was observed. No IgE-mediated reactions to wine have been described, and no grape major allergens have yet been identified. Objective: We describe several severe reactions to grape or wine. We characterized the grape major allergens and tried to identify the allergen in wine. Methods: We collected documented histories of allergic reactions to grape and wine. Grape allergens were identified by means of SDS-PAGE and immunoblotting and purified by means of HPLC. Using amino acid sequencing and mass spectrometry, we identified the family of proteins to which the allergens belong. Cross-reactivity with peach and cherry was evaluated by means of cross-wise inhibition experiments. Results: Eleven patients with reactions to grape and 3 with anaphylactic reactions to wine were recruited. The major allergens were an endochitinase 4A and a lipid-transfer protein (LTP) that was homologous to and cross-reactive with peach LTP. A 24-kd protein homologous to the cherry thaumatin-like allergen was a minor allergen. Endochitinase 4A is very likely the allergen in vino novello and in vino Fragolino. Conclusions: Grape and wine might cause severe allergic reactions in sensitive patients. The major allergens of grape are endochitinase 4A, which is also the allergen of wine, and an LTP cross-reacting with the peach major allergen.

Original languageEnglish
Pages (from-to)350-359
Number of pages10
JournalJournal of Allergy and Clinical Immunology
Volume111
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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Keywords

  • Food hypersensitivity
  • Grape
  • Major allergens
  • Minor allergens
  • Wine

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Pastorello, E. A., Farioli, L., Pravettoni, V., Ortolani, C., Fortunato, D., Gabriella Giuffrida, M., Perono Garoffo, L., Marianna Calamari, A., Brenna, O., & Conti, A. (2003). Identification of grape and wine allergens as an endochitinase 4, a lipid-transfer protein, and a thaumatin. Journal of Allergy and Clinical Immunology, 111(2), 350-359. https://doi.org/10.1067/mai.2003.35