Identification of HIV-specific oligoclonal immunoglobulins in serum of carriers of HIV antibody

N. M. Papadopoulos, R. Costello, M. Ceroni, H. M. Moutsopoulos

Research output: Contribution to journalArticlepeer-review

Abstract

Zone electrophoresis on agarose gel was performed on serum samples from HIV-antibody carriers and negative controls. Nitrocellulose strips precoated with an HIV preparation were then placed on top of the gels and developed by an immunoblotting procedure. A positive reaction was demonstrated between the HIV antigens and the HIV-antibody-positive serum samples with hypergammaglobulinemia and oligoclonal IgG bands. A negative reaction was found between the HIV antigens and HIV-antibody-negative serum samples from a normal person and a patient with monoclonal gammopathy. The presence of oligoclonal IgG bands in the serum of HIV-antibody carriers, and their positive identification with HIV antigens, indicates a specific immune response of the host to the HIV infection and supports the use of oligoclonal IgG bands as markers to follow the course of HIV infection.

Original languageEnglish
Pages (from-to)973-975
Number of pages3
JournalClinical Chemistry
Volume34
Issue number5
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Clinical Biochemistry

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