Identification of HLA-DRα chain residues critical for binding of the toxic shock syndrome toxin superantigen

Paola Panina-Bordignon, Xin Ting Fu, Antonio Lanzavecchia, Robert W. Karr

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Staphylococcal toxic shock syndrome toxin 1 (TSST-1) binds to major histocompatibility complex class II molecules, and the toxin-class II complexes induce proliferation of T cells expressing Vβ2 sequences. To define the residues involved in TSST-1 binding, a set of transfectants expressing 21 HLA-DRα chain mutants were analyzed for their abilities to bind and present TSST-1 and to present an antigenic peptide. Mutations at DRα positions 36 and 39 markedly decreased the ability of the DR7 molecule to bind and present TSST-1 but did not affect the ability to present an antigenic peptide. These data indicate that DRα residues 36 and 39, predicted to be located on an outer loop, are important in the formation of the TSST-1 binding site on DR molecules.

Original languageEnglish
Pages (from-to)1779-1784
Number of pages6
JournalJournal of Experimental Medicine
Volume176
Issue number6
Publication statusPublished - Dec 1 1992

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Superantigens
HLA-DR Antigens
Septic Shock
Peptides
Major Histocompatibility Complex
Binding Sites
Staphylococcal enterotoxin F
T-Lymphocytes
Mutation

ASJC Scopus subject areas

  • Immunology

Cite this

Panina-Bordignon, P., Fu, X. T., Lanzavecchia, A., & Karr, R. W. (1992). Identification of HLA-DRα chain residues critical for binding of the toxic shock syndrome toxin superantigen. Journal of Experimental Medicine, 176(6), 1779-1784.

Identification of HLA-DRα chain residues critical for binding of the toxic shock syndrome toxin superantigen. / Panina-Bordignon, Paola; Fu, Xin Ting; Lanzavecchia, Antonio; Karr, Robert W.

In: Journal of Experimental Medicine, Vol. 176, No. 6, 01.12.1992, p. 1779-1784.

Research output: Contribution to journalArticle

Panina-Bordignon, P, Fu, XT, Lanzavecchia, A & Karr, RW 1992, 'Identification of HLA-DRα chain residues critical for binding of the toxic shock syndrome toxin superantigen', Journal of Experimental Medicine, vol. 176, no. 6, pp. 1779-1784.
Panina-Bordignon, Paola ; Fu, Xin Ting ; Lanzavecchia, Antonio ; Karr, Robert W. / Identification of HLA-DRα chain residues critical for binding of the toxic shock syndrome toxin superantigen. In: Journal of Experimental Medicine. 1992 ; Vol. 176, No. 6. pp. 1779-1784.
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