Identification of HSP-60 as the specific antigen of IgM produced by BRG-lymphoma cells

Luca Musante, Massimo Ulivi, Giovanna Cutrona, Nicholas Chiorazzi, Silvio Roncella, Giovanni Candlano, Manlio Ferrarini

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In previous studies we described a patient with Burkitt's lymphoma and AIDS, whose cells recognized a molecule expressed by normal and malignant breast cells. In the present study, we identified this antigen by two-dimensional (2-D) electrophoresis and Western blotting using the antibody produced by lymphoma cells. The antigen so identified consisted of two clusters of spots with a molecular mass (Mr) of 60 and 50 kDa, respectively. Preparative immobilized pH gradient (IPG) was subsequently used to isolate the clusters of spots of higher molecular masses, from which peptide fragments of approximately 10 aa were separated on reverse-phase chromatography and sequenced. This procedure enabled the identification of the antigen recognized by the lymphoma cells as HSP-60. By means of serological analyses it was possible to identify the lower molecular mass cluster of spots as a molecule related to HSP-60. It is hypothesized that this molecule is a membrane form of HSP-60 that differs from HSP-60 in a COOH terminal portion.

Original languageEnglish
Title of host publicationFrom Genome to Proteome: Advances in the Practice & Application of Proteomics
PublisherWiley Blackwell
Pages512-517
Number of pages6
ISBN (Print)9783527613489, 9783527301546
DOIs
Publication statusPublished - Dec 26 2007

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Keywords

  • Apoptosis
  • Burkitt's lymphoma
  • Heat shock protein
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Musante, L., Ulivi, M., Cutrona, G., Chiorazzi, N., Roncella, S., Candlano, G., & Ferrarini, M. (2007). Identification of HSP-60 as the specific antigen of IgM produced by BRG-lymphoma cells. In From Genome to Proteome: Advances in the Practice & Application of Proteomics (pp. 512-517). Wiley Blackwell. https://doi.org/10.1002/9783527613489.ch67