Identification of human cytomegalovirus isolates by the polymerase chain reaction.

D. Zipeto, E. Silini, M. Parea, E. Percivalle, M. Zavattoni, A. Di Matteo, G. Milanesi, G. Gerna

Research output: Contribution to journalArticle

Abstract

Fifty human cytomegalovirus (HCMV) isolates were recovered from different clinical specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS patients, 20 heart transplant recipients, 1 bone marrow transplant recipient, 2 newborns with congenital HCMV infection and 4 immunocompetent individuals with acute HCMV infection). The isolates were previously identified by immunological methods and then examined for identification by the polymerase chain reaction. In parallel, reference HCMV strains as well as other human members of the Herpesviridae family (reference and wild strains) were examined as controls. Two pairs of primers relevant to the immediate-early and late regions of HCMV DNA, respectively, were used. The DNA amplification product was highly specific; in addition, all fifty HCMV isolates were amplified by both pairs of primers and thus identified as HCMV. These preliminary results show that selected pairs of primers are able to amplify DNA regions conserved enough to allow virus identification among a large number of HCMV strains. In addition, they are highly promising in view of the use of PCR for direct detection of HCMV DNA in clinical samples.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalMicrobiologica
Volume13
Issue number4
Publication statusPublished - Oct 1990

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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