Identification of human pulmonary alkaline phosphatase isoenzymes

Armando Capelli, Carlo G. Cerutti, Mirco Lusuardi, Claudio F. Donner

Research output: Contribution to journalArticlepeer-review

Abstract

An increase of alkaline phosphatase (ALP) activity has been observed in the bronchoalveolar lavage fluid (BALF) of patients affected by pulmonary fibrosis in chronic interstitial lung disorders. To characterize the ALP isoenzymes in such cases, we used gel filtration, agarose gel electrophoresis, heat and amino acid inhibition assays, wheat-germ agglutinin (WGA) precipitation, and an immunoassay specific for the bone-isoform of ALP. Only one anodic band representing a high-molecular-weight isoform of ALP (Mr ~ 2,000 kDa) was observed on electrophoresis of BALF. The inhibition assay results were consistent for a tissue-nonspecific isoenzyme sensitive to a temperature of 56°C (71.9 ± 2.5% inhibition) and to homoarginine (65.7 ± 1.9%), and resistant to L-phenylalanine and L-leucine. Less than 13% of ALP activity was heat-stable. After incubation of BALF specimens with glycosyl- phosphatidylinositol-phospholipase D plus Nonidet P-40, or with phosphatidylinositol-phospholipase C alone, an electrophoretic cathodic band (Mr ~ 220 kDa) appeared near the bone band of a standard serum. With the WGA assay, 84.4 ± 3.3% of ALP precipitated and the band disappeared. After immunoassay for the bone isoform, a mean of less than 5% enzyme activity was measured. We conclude that the ALP found in BALF is a pulmonary isoform of a tissue nonspecific isoenzyme.

Original languageEnglish
Pages (from-to)1448-1452
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume155
Issue number4
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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