Abstract
We analyzed the epitopes and the molecular forms of Tat recognized by the antibodies raised by Tat-toxoid vaccination in both healthy and HIV-infected volunteers. Tat-toxoid - vaccinated healthy volunteer sera reacted predominantly with peptides covering amino acids 1 through 24 and 46 through 60, corresponding to the N-terminus and basic domains of Tat. In contrast, whereas all sera from vaccinated HIV-1 - positive patients reacted with the N-terminus and (with a single exception) with the basic domain, most of these sera also recognized peptides encompassing distinct domains of Tat, particularly the C-terminus (79-86). The sera of vaccinated individuals recognized both monomeric and oligomeric forms of Tat 1 through 86 or of Tat 1 through 101 and also blocked the ability of cell-released extracellular Tat to transactivate the HIV-1 LTR promoter. Synthetic Tat preincubated with sera from vaccinated individuals lost its functional activity as well. This is probably because of its inability to enter the cells as a result of immune complex formation with anti-Tat IgG. These data demonstrate that Tat-toxoid vaccination induces an efficient antibody response blocking the functional activity of Tat.
| Original language | English |
|---|---|
| Pages (from-to) | 47-55 |
| Number of pages | 9 |
| Journal | Journal of Acquired Immune Deficiency Syndromes |
| Volume | 33 |
| Issue number | 1 |
| Publication status | Published - May 1 2003 |
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Keywords
- Epitope mapping
- HIV
- Humoral response
- Kaposi sarcoma
- Tat
- Vaccine
ASJC Scopus subject areas
- Virology
- Immunology
Cite this
Identification of immunodominant epitopes in inactivated Tat-vaccinated healthy and HIV-1-infected volunteers. / Noonan, Douglas M.; Gringeri, Alessandro; Meazza, Raffaella; Rosso, Ombretta; Mazza, Stefania; Muça-Perja, Myrvet; Le Buanec, Hélène; Accolla, Roberto S.; Albini, Adriana; Ferrini, Silvano.
In: Journal of Acquired Immune Deficiency Syndromes, Vol. 33, No. 1, 01.05.2003, p. 47-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of immunodominant epitopes in inactivated Tat-vaccinated healthy and HIV-1-infected volunteers
AU - Noonan, Douglas M.
AU - Gringeri, Alessandro
AU - Meazza, Raffaella
AU - Rosso, Ombretta
AU - Mazza, Stefania
AU - Muça-Perja, Myrvet
AU - Le Buanec, Hélène
AU - Accolla, Roberto S.
AU - Albini, Adriana
AU - Ferrini, Silvano
PY - 2003/5/1
Y1 - 2003/5/1
N2 - We analyzed the epitopes and the molecular forms of Tat recognized by the antibodies raised by Tat-toxoid vaccination in both healthy and HIV-infected volunteers. Tat-toxoid - vaccinated healthy volunteer sera reacted predominantly with peptides covering amino acids 1 through 24 and 46 through 60, corresponding to the N-terminus and basic domains of Tat. In contrast, whereas all sera from vaccinated HIV-1 - positive patients reacted with the N-terminus and (with a single exception) with the basic domain, most of these sera also recognized peptides encompassing distinct domains of Tat, particularly the C-terminus (79-86). The sera of vaccinated individuals recognized both monomeric and oligomeric forms of Tat 1 through 86 or of Tat 1 through 101 and also blocked the ability of cell-released extracellular Tat to transactivate the HIV-1 LTR promoter. Synthetic Tat preincubated with sera from vaccinated individuals lost its functional activity as well. This is probably because of its inability to enter the cells as a result of immune complex formation with anti-Tat IgG. These data demonstrate that Tat-toxoid vaccination induces an efficient antibody response blocking the functional activity of Tat.
AB - We analyzed the epitopes and the molecular forms of Tat recognized by the antibodies raised by Tat-toxoid vaccination in both healthy and HIV-infected volunteers. Tat-toxoid - vaccinated healthy volunteer sera reacted predominantly with peptides covering amino acids 1 through 24 and 46 through 60, corresponding to the N-terminus and basic domains of Tat. In contrast, whereas all sera from vaccinated HIV-1 - positive patients reacted with the N-terminus and (with a single exception) with the basic domain, most of these sera also recognized peptides encompassing distinct domains of Tat, particularly the C-terminus (79-86). The sera of vaccinated individuals recognized both monomeric and oligomeric forms of Tat 1 through 86 or of Tat 1 through 101 and also blocked the ability of cell-released extracellular Tat to transactivate the HIV-1 LTR promoter. Synthetic Tat preincubated with sera from vaccinated individuals lost its functional activity as well. This is probably because of its inability to enter the cells as a result of immune complex formation with anti-Tat IgG. These data demonstrate that Tat-toxoid vaccination induces an efficient antibody response blocking the functional activity of Tat.
KW - Epitope mapping
KW - HIV
KW - Humoral response
KW - Kaposi sarcoma
KW - Tat
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=0038034441&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038034441&partnerID=8YFLogxK
M3 - Article
C2 - 12792355
AN - SCOPUS:0038034441
VL - 33
SP - 47
EP - 55
JO - Journal of Acquired Immune Deficiency Syndromes
JF - Journal of Acquired Immune Deficiency Syndromes
SN - 1525-4135
IS - 1
ER -