Identification of key regions and genes important in the pathogenesis of Sézary syndrome by combining genomic and expression microarrays

Elisabetta Caprini, Cristina Cristofoletti, Diego Arcelli, Paolo Fadda, Mauro Helmer Citterich, Francesca Sampogna, Armando Magrelli, Federica Censi, Paola Torreri, Marina Frontani, Enrico Scala, Maria Cristina Picchio, Paola Temperani, Alessandro Monopoli, Giuseppe Alfonso Lombardo, Domenica Taruscio, Maria Grazia Narducci, Giandomenico Russo

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we used single nucleotide polymorphism and comparative genomic hybridization array to study DNA copy number changes and loss of heterozygosity for 28 patients affected by Sézary syndrome (SS), a rare form of cutaneous T-cell lymphoma (CTCL). Our data identified, further confirming previous studies, recurrent losses of 17p13.2-p11.2 and 10p12.1-q26.3 occurring in 71% and 68% of cases, respectively; common gains were detected for 17p11.2-q25.3 (64%) and chromosome 8/8q (50%). Moreover, we identified novel genomic lesions recurring in >30% of tumors: loss of 9q13-q21.33 and gain of 10p15.3-10p12.2. Individual chromosomal aberrations did not show a significant correlation with prognosis; however, when more than three recurrent chromosomal alterations (gain or loss) were considered, a statistical association was observed using Kaplan-Meier survival analysis. Integrating mapping and transcriptional data, we were able to identify a total of 113 deregulated transcripts in aberrant chromosomal regions that included cancer-related genes such as members of the NF-κB pathway (BAG4, BTRC, NKIRAS2, PSMD3, and TRAF2) that might explain its constitutive activation in CTCL. Matching this list of genes with those discriminating patients with different survival times, we identify several common candidates that might exert critical roles in SS, such as BUB3 and PIP5K1B. Altogether, our study confirms and maps more precisely the regions of gain and loss and, combined to transcriptional profiles, suggests a novel set of genes of potential interest in SS.

Original languageEnglish
Pages (from-to)8438-8446
Number of pages9
JournalCancer Research
Volume69
Issue number21
DOIs
Publication statusPublished - Nov 1 2009

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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