TY - JOUR
T1 - Identification of L-methionine oxidation products in tripeptides, in Met-enkephalin and in the bovine basic pancreatic trypsin inhibitor
T2 - 1H and 13C NMR study
AU - Fruttero, R.
AU - Amiconi, G.
AU - Ascoli, F.
AU - Bolognesi, M.
AU - Ascenzi, P.
PY - 1995
Y1 - 1995
N2 - L-methionine (Met) oxidation products in tripeptides, in Met-enkephalin and in the bovine basic pancreatic trypsin inhibitor have been identified by 1H and 13C NMR spectroscopy. The oxidation of Met residues by stoichiometric amounts of chloramine-B, H2O2 and I2 yields a mixture of L-methionine sulfoxide (Met(O)) and dehydro-L-methionine (DH-Met), Met(O) and DH-Met, respectively, at pH 7.0 and 25.0°C. The formation of DH-Met occurs only if the amino-group of Met is not derivatized. The analysis of 1H and 13C NMR spectra allows us to quantitate Met oxidation products, to ascertain the relative proportion of the R and S forms of Met(O) and DH-Met, and to reveal the presence of a Met residue at the N-terminal position in peptides and proteins.
AB - L-methionine (Met) oxidation products in tripeptides, in Met-enkephalin and in the bovine basic pancreatic trypsin inhibitor have been identified by 1H and 13C NMR spectroscopy. The oxidation of Met residues by stoichiometric amounts of chloramine-B, H2O2 and I2 yields a mixture of L-methionine sulfoxide (Met(O)) and dehydro-L-methionine (DH-Met), Met(O) and DH-Met, respectively, at pH 7.0 and 25.0°C. The formation of DH-Met occurs only if the amino-group of Met is not derivatized. The analysis of 1H and 13C NMR spectra allows us to quantitate Met oxidation products, to ascertain the relative proportion of the R and S forms of Met(O) and DH-Met, and to reveal the presence of a Met residue at the N-terminal position in peptides and proteins.
UR - http://www.scopus.com/inward/record.url?scp=0029550647&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029550647&partnerID=8YFLogxK
M3 - Article
C2 - 7542960
AN - SCOPUS:0029550647
VL - 35
SP - 861
EP - 874
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
SN - 1039-9712
IS - 4
ER -