Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Mara Mancini; Anna Maria Lena; Eleonora Candi

doi:10.1007/978-1-0716-0648-3_15

Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Mara Mancini, Anna Maria Lena, Eleonora Candi

Istituto Dermopatico dell'Immacolata , IDI

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	175-185
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-0648-3_15
Publication status	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2154
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Fluorescence in situ hybridization
In situ hybridization
Keratinocytes
Long non coding RNA
Noncoding RNA
Skin

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-0648-3_15

Cite this

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abstract = "In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.",

keywords = "Fluorescence in situ hybridization, In situ hybridization, Keratinocytes, Long non coding RNA, Noncoding RNA, Skin",

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AU - Mancini, Mara

AU - Lena, Anna Maria

AU - Candi, Eleonora

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AB - In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

KW - Fluorescence in situ hybridization

KW - In situ hybridization

KW - Keratinocytes

KW - Long non coding RNA

KW - Noncoding RNA

KW - Skin

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ER -

Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

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