Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Mara Mancini, Anna Maria Lena, Eleonora Candi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages175-185
Number of pages11
DOIs
Publication statusPublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2154
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Fluorescence in situ hybridization
  • In situ hybridization
  • Keratinocytes
  • Long non coding RNA
  • Noncoding RNA
  • Skin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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