Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Mara Mancini; Anna Maria Lena; Eleonora Candi

doi:10.1007/978-1-0716-0648-3_15

Identification of Long Noncoding RNA by In Situ Hybridization Approaches

Mara Mancini, Anna Maria Lena, Eleonora Candi

Istituto Dermopatico dell'Immacolata , IDI

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	175-185
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-0716-0648-3_15
Publication status	Published - 2020

Publication series

Name	Methods in Molecular Biology
Volume	2154
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Fluorescence in situ hybridization
In situ hybridization
Keratinocytes
Long non coding RNA
Noncoding RNA
Skin

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-0648-3_15

Fingerprint Dive into the research topics of 'Identification of Long Noncoding RNA by In Situ Hybridization Approaches'. Together they form a unique fingerprint.

Cite this

@inbook{45ac92d4d37f4ad0b0d399c782cc0682,

title = "Identification of Long Noncoding RNA by In Situ Hybridization Approaches",

abstract = "In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.",

keywords = "Fluorescence in situ hybridization, In situ hybridization, Keratinocytes, Long non coding RNA, Noncoding RNA, Skin",

author = "Mara Mancini and Lena, {Anna Maria} and Eleonora Candi",

year = "2020",

doi = "10.1007/978-1-0716-0648-3_15",

language = "English",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "175--185",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Identification of Long Noncoding RNA by In Situ Hybridization Approaches

AU - Mancini, Mara

AU - Lena, Anna Maria

AU - Candi, Eleonora

PY - 2020

Y1 - 2020

N2 - In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

AB - In situ hybridization (ISH) and fluorescence in situ hybridization (FISH) techniques enable us to detect the expression of a specific RNA in fixed cells or tissue sections. Here, we describe in detail two procedures adjusted to reveal specifically lncRNAs in normal human keratinocytes and in skin tissue samples. Examples of the results obtained by the two different approaches are also shown.

KW - Fluorescence in situ hybridization

KW - In situ hybridization

KW - Keratinocytes

KW - Long non coding RNA

KW - Noncoding RNA

KW - Skin

UR - http://www.scopus.com/inward/record.url?scp=85083811908&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85083811908&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-0648-3_15

DO - 10.1007/978-1-0716-0648-3_15

M3 - Chapter

C2 - 32314217

AN - SCOPUS:85083811908

T3 - Methods in Molecular Biology

SP - 175

EP - 185

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -