Identification of merkel cell polyomavirus in the lower respiratory tract of Italian patients

Muhammed Babakir-Mina, Massimo Ciccozzi, Alessandra Lo Presti, Francesco Greco, Carlo Federico Perno, Marco Ciotti

Research output: Contribution to journalArticle

Abstract

Merkel cell polyomavirus (MCPyV) has been found to be integrated monoclonally in a rare skin cancer named Merkel cell carcinoma. More recently, MCPyV has been detected in the upper respiratory tract of pediatric and adult patients. However, the mode of transmission and pathogenic role ofMCPyV in the respiratory system has not been determined. In this study, MCPyV was sought in the lower respiratory tract of adult patients admitted to the hospital. MCPyV DNA was detected in 15 (17.24%) out of 87 lower respiratory tract samples. Most of the patients with MCPyV were over 50 years old. Nucleotide sequence of the t-antigen of MCPyV identified in respiratory secretions showed a homology to those found in Merkel cell carcinoma. In addition, phylogenetic analysis undertaken on the t-antigen sequences of Italian isolates and other MCPyVs identified in healthy and cancer tissues showed that all these isolates belonged to the same clade. Selective pressure analysis for the tantigen revealed the presence of five sites under positive selection (ω=4.3), with a posterior probabilities above 0.99. The α parameter of the gamma distribution was 0.01, showing that this distribution has a characteristic L-shape and suggesting a strong nucleotide substitution rate heterogeneity across sites. This study shows that MCPyV can infect the lower respiratory tract, but further investigations are needed to define its pathogenic role in respiratory diseases.

Original languageEnglish
Pages (from-to)505-509
Number of pages5
JournalJournal of Medical Virology
Volume82
Issue number3
DOIs
Publication statusPublished - 2010

Fingerprint

Merkel cell polyomavirus
Respiratory System
Merkel Cell Carcinoma
Antigens
Skin Neoplasms
Nucleotides
Pediatrics

Keywords

  • Lower respiratory tract
  • MC polyomavirus
  • Novel polyomaviruses
  • Phylogenetic analysis
  • Respiratory diseases

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Identification of merkel cell polyomavirus in the lower respiratory tract of Italian patients. / Babakir-Mina, Muhammed; Ciccozzi, Massimo; Lo Presti, Alessandra; Greco, Francesco; Perno, Carlo Federico; Ciotti, Marco.

In: Journal of Medical Virology, Vol. 82, No. 3, 2010, p. 505-509.

Research output: Contribution to journalArticle

Babakir-Mina, Muhammed ; Ciccozzi, Massimo ; Lo Presti, Alessandra ; Greco, Francesco ; Perno, Carlo Federico ; Ciotti, Marco. / Identification of merkel cell polyomavirus in the lower respiratory tract of Italian patients. In: Journal of Medical Virology. 2010 ; Vol. 82, No. 3. pp. 505-509.
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