Identification of microRNA expression patterns and definition of a microRNA/mRNA regulatory network in distinct molecular groups of multiple myeloma

Marta Lionetti, Marta Biasiolo, Luca Agnelli, Katia Todoerti, Laura Mosca, Sonia Fabris, Gabriele Sales, Giorgio Lambertenghi Deliliers, Silvio Bicciato, Luigia Lombardi, Stefania Bortoluzzi, Antonino Neri

Research output: Contribution to journalArticle

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Abstract

To date, little evidence of miRNA expression/deregulation in multiple myeloma has been reported. To characterize miRNA in the context of the major multiple myeloma molecular types, we generated miRNA expression profiles of highly purified malignant plasma cells from 40 primary tumors. Furthermore, transcriptional profiles, available for all patients, were used to investigate the occurrence of miRNA/predicted target mRNA pair anticorrelations, and the miRNA and genome-wide DNA data were integrated in a subset of patients to evaluate the influence of allelic imbalances on miRNA expression. Differential miRNA expression patterns were identified, which were mainly associated with the major IGH translocations; particularly, t(4;14) patients showed specific overexpression of let-7e, miR-125a-5p, and miR-99b belonging to a cluster at 19q13.33. The occurrence of other lesions (ie, 1q gain, 13q and 17p deletions, and hyperdiploidy) was slightly characterized by specific miRNA signatures. Furthermore, the occurrence of several allelic imbalances or loss of heterozygosity was found significantly associated with the altered expression of miRNAs located in the involved regions, such as let-7b at 22q13.31 or miR-140-3p at 16q22. Finally, the integrative analysis based on computational target prediction and miRNA/ mRNA profiling defined a network of putative functional miRNA-target regulatory relations supported by expression data.

Original languageEnglish
JournalBlood
Volume114
Issue number25
DOIs
Publication statusPublished - Dec 10 2009

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Multiple Myeloma
MicroRNAs
Messenger RNA
Allelic Imbalance
Loss of Heterozygosity
Polyploidy
Deregulation
Plasma Cells
Tumors
Genes
Genome
Plasmas
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Immunology
  • Hematology

Cite this

Identification of microRNA expression patterns and definition of a microRNA/mRNA regulatory network in distinct molecular groups of multiple myeloma. / Lionetti, Marta; Biasiolo, Marta; Agnelli, Luca; Todoerti, Katia; Mosca, Laura; Fabris, Sonia; Sales, Gabriele; Deliliers, Giorgio Lambertenghi; Bicciato, Silvio; Lombardi, Luigia; Bortoluzzi, Stefania; Neri, Antonino.

In: Blood, Vol. 114, No. 25, 10.12.2009.

Research output: Contribution to journalArticle

Lionetti, Marta ; Biasiolo, Marta ; Agnelli, Luca ; Todoerti, Katia ; Mosca, Laura ; Fabris, Sonia ; Sales, Gabriele ; Deliliers, Giorgio Lambertenghi ; Bicciato, Silvio ; Lombardi, Luigia ; Bortoluzzi, Stefania ; Neri, Antonino. / Identification of microRNA expression patterns and definition of a microRNA/mRNA regulatory network in distinct molecular groups of multiple myeloma. In: Blood. 2009 ; Vol. 114, No. 25.
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