Identification of miR-187 and miR-182 as biomarkers of early diagnosis and prognosis in patients with prostate cancer treated with radical prostatectomy

Irene Casanova-Salas, José Rubio-Briones, Ana Calatrava, Caterina Mancarella, Esther Masiá, Juan Casanova, Antonio Fernández-Serra, Luis Rubio, Miguel Ramírez-Backhaus, Ana Armiñán, José Domínguez-Escrig, Francisco Martínez, Zaida García-Casado, Katia Scotlandi, María J. Vicent, José Antonio López-Guerrero

Research output: Contribution to journalArticle

Abstract

Purpose miRNAs are noncoding RNAs that negatively regulate target mRNA gene expression. Aberrant miRNA expression is associated with prostate cancer pathogenesis. We identified miRNAs as potential biomarkers for prostate cancer diagnosis and prognosis. Materials and Methods Total RNA was obtained from 10 normal prostate and 50 prostate cancer samples, and analyzed using the GeneChip® miRNA 2.0 Array. At a median followup of 92 months (range 2 to 189) an independent cohort of 273 paraffin embedded prostate cancer samples was used for validation by quantitative reverse transcriptase-polymerase chain reaction. Another 92 urine samples from patients undergoing prostate biopsy were evaluated for these miRNAs. Results miR-182 and 187, the miRNAs most differentially expressed between normal and tumor tissue, were selected for further validation. miR-187 inversely correlated with cT (p = 0.125) and pT (p = 0.0002) stages, Gleason score (p = 0.003) and TMPRSS2-ERG status (p = 0.003). The log rank test showed associations of miR-182 with biochemical (p = 0.026) and clinical (p = 0.043) progression-free survival, as also noted on multivariate analysis. A significant independent improvement in the definition of risk of progression was achieved by combining miR-182 expression with Gleason score (p

Original languageEnglish
Pages (from-to)252-259
Number of pages8
JournalJournal of Urology
Volume192
Issue number1
DOIs
Publication statusPublished - 2014

Keywords

  • biological markers
  • diagnosis
  • microRNAs
  • prognosis
  • prostatic neoplasms

ASJC Scopus subject areas

  • Urology
  • Medicine(all)

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    Casanova-Salas, I., Rubio-Briones, J., Calatrava, A., Mancarella, C., Masiá, E., Casanova, J., Fernández-Serra, A., Rubio, L., Ramírez-Backhaus, M., Armiñán, A., Domínguez-Escrig, J., Martínez, F., García-Casado, Z., Scotlandi, K., Vicent, M. J., & López-Guerrero, J. A. (2014). Identification of miR-187 and miR-182 as biomarkers of early diagnosis and prognosis in patients with prostate cancer treated with radical prostatectomy. Journal of Urology, 192(1), 252-259. https://doi.org/10.1016/j.juro.2014.01.107