Identification of novel alternatively-spliced mRNA isoforms of metabotropic glutamate receptor 6 gene in rat and human retina

Alessandra Valerio, Sergio Ferraboli, Marta Paterlini, PierFranco Spano, Sergio Barlati

Research output: Contribution to journalArticle

Abstract

Novel splice variants of metabotropic glutamate receptor type 6 (mGlu6 receptor) were identified by reverse transcription-polymerase chain reaction (RT-PCR) amplification and sequence analysis of rat and human retina cDNAs. The new rat mGlu6 receptor mRNA isoform is characterized by an additional exon of 88 nucleotides containing an in frame stop codon, thus predicting the expression of a truncated protein of 508 amino acids. The human retina was found to express two different mGlu6 receptor mRNA variants: one lacking 97 nucleotides from exon 6, the other including five nucleotides of intron 5. These mRNAs would encode truncated receptors of 425 and 405 amino acids, respectively. Both in rats and in humans, the truncated mGlu6 receptor proteins would comprise the extracellular domain but lack the transmembrane and intracellular portion of the receptor, thus possibly acting as retinal soluble receptors for glutamate. Though generated by different patterns of alternative splicing, the inter-species conservation of truncated mGlu receptor molecules strongly suggest their relevance in the regulatory network of glutamatergic neurotransmission.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalGene
Volume262
Issue number1-2
DOIs
Publication statusPublished - Jan 10 2001

Keywords

  • Glutamatergic neurotransmission
  • GTP-binding protein-coupled receptors
  • mRNA
  • Reverse transcription-polymerase chain reaction
  • Soluble receptors

ASJC Scopus subject areas

  • Genetics

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