Identification of Novel Chimpanzee MHC Class I and II Alleles Using an Improved Sequence-Based Typing Strategy

Laura Caggiari, Maria Paola Simula, Alessandra Marzotto, Masaaki Shiina, Barbara Rehermann, Valli De Re

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The chimpanzee (Pan troglodytes) is a valuable model for the study of infection with hepatitis C virus and hepatitis B virus, to which only humans and chimpanzees are susceptible. Because the cellular immune response plays a crucial role in host defense against these viruses, the analysis of major histocompatibility complex (MHC) (Patr) class I and II allele diversity in chimpanzees is essential for immune response analysis and vaccine development. In the present study, we report a novel, rapid, and sensitive sequence-based typing strategy to identify polymorphisms of the principal Patr class I (Patr-A and Patr-B) and Patr class II (Patr-DQB1 and Patr-DRB1) alleles. Using this method we identified seven novel Patr alleles in 17 chimpanzees, one of them present in 3 chimpanzees. Furthermore, we detected heterozygosity more rapidly and with higher sensitivity than was done with previous techniques that were based on reverse transcription and amplification of messenger RNA followed by molecular cloning and sequencing.

Original languageEnglish
Pages (from-to)63-72
Number of pages10
JournalHuman Immunology
Volume67
Issue number1-2
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Pan troglodytes
Major Histocompatibility Complex
Alleles
Molecular Cloning
Hepatitis B virus
Cellular Immunity
Hepacivirus
Reverse Transcription
Vaccines
Viruses
Messenger RNA
Infection

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Identification of Novel Chimpanzee MHC Class I and II Alleles Using an Improved Sequence-Based Typing Strategy. / Caggiari, Laura; Simula, Maria Paola; Marzotto, Alessandra; Shiina, Masaaki; Rehermann, Barbara; De Re, Valli.

In: Human Immunology, Vol. 67, No. 1-2, 01.2006, p. 63-72.

Research output: Contribution to journalArticle

Caggiari, Laura ; Simula, Maria Paola ; Marzotto, Alessandra ; Shiina, Masaaki ; Rehermann, Barbara ; De Re, Valli. / Identification of Novel Chimpanzee MHC Class I and II Alleles Using an Improved Sequence-Based Typing Strategy. In: Human Immunology. 2006 ; Vol. 67, No. 1-2. pp. 63-72.
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