Identification of novel plasma glycosylation-associated markers of aging

Mariangela Catera, Vincenzo Borelli, Nadia Malagolini, Mariella Chiricolo, Giulia Venturi, Celso Reis, Hugo Osoquot;rio, Provvidenza Maria Abruzzo, Miriam Capri, D. Monti, R. Ostan, Claudio Franceschi, Fabio Dall'Olio

Research output: Contribution to journalArticle

Abstract

The pro- or anti-inflammatory activities of immunoglobulins G (IgGs) are controlled by the structure of the glycan N-linked to Asn297 of their heavy chain. The age-associated low grade inflammation (inflammaging) is associated with increased plasmatic levels of agalactosylated IgGs terminating with N-acetylglucosamine (IgG-G0) whose biogenesis has not been fully explained. Although the biosynthesis of glycans is in general mediated by glycosyltransferases associated with internal cell membranes, the extracellular glycosylation of circulating glycoproteins mediated by plasmatic glycosyltransferases has been recently demonstrated. In this study we have investigated the relationship between plasmatic glycosyltransferases, IgG glycosylation and inflammatory and aging markers. In cohorts of individuals ranging from infancy to centenarians we determined the activity of plasmatic β4 galactosyltransferase(s) (B4GALTs) and of a2,6-sialyltransferase ST6GAL1, the glycosylation of IgG, the GlycoAge test (a glycosylation-based marker of aging) and the plasma level of inflammatory and liver damage markers. Our results show that: 1) plasmatic B4GALTs activity is a new marker of aging, showing a linear increase throughout the whole age range. 2) plasmatic ST6GAL1 was high only in children and in people above 80, showing a quadratic relationship with age. 3) Neither plasmatic glycosyltransferase correlated with markers of liver damage. 4) plasmatic ST6GAL1 showed a positive association with acute phase proteins in offspring of short lived parents, but not in centenarians or in their offspring. 5) Although the glycosylation of IgGs was not correlated with the level of the two plasmatic glycosyltransferases, it showed progressive age-associated changes consistent with a shift toward a pro-inflammatory glycotype.

Original languageEnglish
Pages (from-to)7455-7468
Number of pages14
JournalOncotarget
Volume7
Issue number7
DOIs
Publication statusPublished - 2016

Fingerprint

Glycosyltransferases
Glycosylation
Immunoglobulin G
Polysaccharides
Sialyltransferases
Galactosyltransferases
Acetylglucosamine
Acute-Phase Proteins
Liver
Glycoproteins
Anti-Inflammatory Agents
Parents
Cell Membrane
Inflammation

Keywords

  • Antibody glycosylation
  • Gerotarget
  • Inflammaging
  • Plasma galactosyltransferases
  • Plasma sialyltransferases
  • Soluble glycosyltransferases

ASJC Scopus subject areas

  • Oncology

Cite this

Catera, M., Borelli, V., Malagolini, N., Chiricolo, M., Venturi, G., Reis, C., ... Dall'Olio, F. (2016). Identification of novel plasma glycosylation-associated markers of aging. Oncotarget, 7(7), 7455-7468. https://doi.org/10.18632/oncotarget.7059

Identification of novel plasma glycosylation-associated markers of aging. / Catera, Mariangela; Borelli, Vincenzo; Malagolini, Nadia; Chiricolo, Mariella; Venturi, Giulia; Reis, Celso; Osoquot;rio, Hugo; Abruzzo, Provvidenza Maria; Capri, Miriam; Monti, D.; Ostan, R.; Franceschi, Claudio; Dall'Olio, Fabio.

In: Oncotarget, Vol. 7, No. 7, 2016, p. 7455-7468.

Research output: Contribution to journalArticle

Catera, M, Borelli, V, Malagolini, N, Chiricolo, M, Venturi, G, Reis, C, Osoquot;rio, H, Abruzzo, PM, Capri, M, Monti, D, Ostan, R, Franceschi, C & Dall'Olio, F 2016, 'Identification of novel plasma glycosylation-associated markers of aging', Oncotarget, vol. 7, no. 7, pp. 7455-7468. https://doi.org/10.18632/oncotarget.7059
Catera M, Borelli V, Malagolini N, Chiricolo M, Venturi G, Reis C et al. Identification of novel plasma glycosylation-associated markers of aging. Oncotarget. 2016;7(7):7455-7468. https://doi.org/10.18632/oncotarget.7059
Catera, Mariangela ; Borelli, Vincenzo ; Malagolini, Nadia ; Chiricolo, Mariella ; Venturi, Giulia ; Reis, Celso ; Osoquot;rio, Hugo ; Abruzzo, Provvidenza Maria ; Capri, Miriam ; Monti, D. ; Ostan, R. ; Franceschi, Claudio ; Dall'Olio, Fabio. / Identification of novel plasma glycosylation-associated markers of aging. In: Oncotarget. 2016 ; Vol. 7, No. 7. pp. 7455-7468.
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