Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries

Irene Cortese, Rosalba Tafi, Luigi M E Grimaldi, Gianvito Martino, Alfredo Nicosia, Riccardo Cortese

Research output: Contribution to journalArticle

Abstract

The study of the origin and pathogenetic relevance of the oligoclonal antibodies present in the cerebrospinal fluid (CSF) of multiple sclerosis (MS) patients has been hampered by a lack of specific ligands. We recently reported a general strategy, based on phage-displayed random peptide libraries, to identify ligands for disease-specific antibodies even in the absence of any information on the nature of the pathologic antigen. With this procedure, we identified several peptides specifically recognized by antibodies present in the CSF of MS patients. Using these peptides as reagents, we demonstrated that they mimic different natural epitopes and react with antibodies enriched in the CSF of MS patients. Antibodies recognizing the selected peptides are commonly found with equal frequency in the sera of MS patients and of normal individuals. In contrast, the repertoire of CSF antibodies appears to be individual-specific and is probably the result of a nonspecific immunodysregulation rather than a stereotyped response to a single antigen/agent.

Original languageEnglish
Pages (from-to)11063-11067
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number20
DOIs
Publication statusPublished - Oct 1 1996

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Bacteriophages
Multiple Sclerosis
Cerebrospinal Fluid
Peptides
Antibodies
Ligands
Antigens
Peptide Library
Epitopes
Serum

ASJC Scopus subject areas

  • Genetics
  • General

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Identification of peptides specific for cerebrospinal fluid antibodies in multiple sclerosis by using phage libraries. / Cortese, Irene; Tafi, Rosalba; Grimaldi, Luigi M E; Martino, Gianvito; Nicosia, Alfredo; Cortese, Riccardo.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 20, 01.10.1996, p. 11063-11067.

Research output: Contribution to journalArticle

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