Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition

Giulia Fontemaggi; Teresa Bellissimo; Sara Donzelli; Ilaria Iosue; Barbara Benassi; Giorgio Bellotti; Giovanni Blandino; Francesco Fazi

doi:10.1080/15476286.2015.1044194

Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition

Giulia Fontemaggi, Teresa Bellissimo, Sara Donzelli, Ilaria Iosue, Barbara Benassi, Giorgio Bellotti, Giovanni Blandino, Francesco Fazi

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

Treatment of leukemia cells with 1, 25-dihydroxyvitamin D₃ may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.

Original language	English
Pages (from-to)	690-700
Number of pages	11
Journal	RNA Biology
Volume	12
Issue number	7
DOIs	https://doi.org/10.1080/15476286.2015.1044194
Publication status	Published - Jan 1 2015

Keywords

AML
MicroRNAs
Myeloid differentiation
PLK1
Ribosomal/polysomal fractions

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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Identification of post-transcriptional regulatory networks during myeloblast-to-monocyte differentiation transition. / Fontemaggi, Giulia; Bellissimo, Teresa; Donzelli, Sara; Iosue, Ilaria; Benassi, Barbara; Bellotti, Giorgio; Blandino, Giovanni; Fazi, Francesco.

In: RNA Biology, Vol. 12, No. 7, 01.01.2015, p. 690-700.

Research output: Contribution to journal › Article › peer-review

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abstract = "Treatment of leukemia cells with 1, 25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.",

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