Abstract
Treatment of leukemia cells with 1, 25-dihydroxyvitamin D3 may overcome their differentiation block and lead to the transition from myeloblasts to monocytes. To identify microRNA-mRNA networks relevant for myeloid differentiation, we profiled the expression of mRNAs and microRNAs associated to the low- and high-density ribosomal fractions in leukemic cells and in their differentiated monocytic counterpart. Intersection between mRNAs shifted across the fractions after treatment with putative target genes of modulated microRNAs showed a series of molecular networks relevant for the monocyte cell fate determination, as for example the post-transcriptional regulation of the Polo-like kinase 1 (PLK1) by miR-22–3p and let-7e-5p.
Original language | English |
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Pages (from-to) | 690-700 |
Number of pages | 11 |
Journal | RNA Biology |
Volume | 12 |
Issue number | 7 |
DOIs | |
Publication status | Published - Jan 1 2015 |
Keywords
- AML
- MicroRNAs
- Myeloid differentiation
- PLK1
- Ribosomal/polysomal fractions
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology