Identification of RASSF8 as a candidate lung tumor suppressor gene

F. S. Falvella, G. Manenti, M. Spinola, C. Pignatiello, B. Conti, U. Pastorino, T. A. Dragani

Research output: Contribution to journalArticlepeer-review

Abstract

The RASSF8 gene, which maps close to the KRAS2 gene, contains a RAS-associated domain and encodes a protein that is evolutionarily conserved from fish to humans. Analysis of the RASSF8 transcript revealed a complex expression pattern of 5′-UTR mRNA isoforms in normal lung and in lung adenocarcinomas (ADCAs), with no apparent differences. However, RASSF8 gene transcript levels were ∼seven-fold-lower in lung ADCAs as compared to normal lung tissue. Expression of RASSF8 protein by transfected lung cancer cells led to inhibition of anchorage-independent growth in soft agar in A549 cells and reduction of clonogenic activity in NCI-H520 cells. These results raise the possibility protein encoded by RASSF8 is a novel tumor suppressor for lung cancer.

Original languageEnglish
Pages (from-to)3934-3938
Number of pages5
JournalOncogene
Volume25
Issue number28
DOIs
Publication statusPublished - Jun 29 2006

Keywords

  • Cancer progression
  • KRAS
  • Lung cancer
  • RAS genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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