TY - JOUR
T1 - Identification of specific plasma proteins determining the agarose gel electrophoresis by the immunosubtraction technique
AU - Merlini, G.
AU - Pavesi, F.
AU - Carini, A.
AU - Zorzoli, I.
AU - Valentini, O.
AU - Aguzzi, F.
PY - 1983
Y1 - 1983
N2 - The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, β-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
AB - The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, β-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
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M3 - Article
C2 - 6663244
VL - 21
SP - 841
EP - 844
JO - Journal of Clinical Chemistry and Clinical Biochemistry
JF - Journal of Clinical Chemistry and Clinical Biochemistry
SN - 0340-076X
IS - 12
ER -