The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, β-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
|Number of pages||4|
|Journal||Journal of Clinical Chemistry and Clinical Biochemistry|
|Publication status||Published - 1983|
ASJC Scopus subject areas
- Clinical Biochemistry