Identification of specific plasma proteins determining the agarose gel electrophoresis by the immunosubtraction technique

G. Merlini, F. Pavesi, A. Carini, I. Zorzoli, O. Valentini, F. Aguzzi

Research output: Contribution to journalArticlepeer-review

Abstract

The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, β-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.

Original languageEnglish
Pages (from-to)841-844
Number of pages4
JournalJournal of Clinical Chemistry and Clinical Biochemistry
Volume21
Issue number12
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Identification of specific plasma proteins determining the agarose gel electrophoresis by the immunosubtraction technique'. Together they form a unique fingerprint.

Cite this