Identification of specific plasma proteins determining the agarose gel electrophoresis by the immunosubtraction technique

The immunosubtraction technique was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipitated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anode): prealbumin, albumin, α-lipoproteins, α₁-antitrypsin, Gc-globulins, α₂-macroglobulin, haptoglobin, fibronectin, transferrin, β-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.