Identifizierung spezifischer Pläsmaproteine mit Agarosegel Elektrophorese durch Immunsubstraktions-Technik

The immunosubtraction techniqiie was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anöde): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, ß-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.