Abstract
The immunosubtraction techniqiie was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anöde): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, ß-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.
Translated title of the contribution | Identification of Specific Plasma Proteins Determining the Agarose Gel Electrophoresis by the Immunosubtraction Technique |
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Original language | Undefined/Unknown |
Pages (from-to) | 841-844 |
Number of pages | 4 |
Journal | Clinical Chemistry and Laboratory Medicine |
Volume | 21 |
Issue number | 12 |
DOIs | |
Publication status | Published - 1983 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical