Identifizierung spezifischer Pläsmaproteine mit Agarosegel Elektrophorese durch Immunsubstraktions-Technik

Translated title of the contribution: Identification of Specific Plasma Proteins Determining the Agarose Gel Electrophoresis by the Immunosubtraction Technique

G. Merlini, Irene Zorzoli

Research output: Contribution to journalArticlepeer-review

Abstract

The immunosubtraction techniqiie was used to identify the proteins in electrophoretically separated plasma. The technique consists of forcing the plasma through a layer of monospecific antibody purified by electrophoresis; the specific protein is thereby precipated and is consequently absent from the subsequent electrophoretogram. This technique was successfully applied to the following proteins (in order of distance from the anöde): prealbumin, albumin, α-lipoproteins, α1-antitrypsin, Gc-globulins, α2-macroglobulin, haptoglobin, fibronectin, transferrin, ß-lipoproteins, C3, fibrinogen. The method may be applied to the study of protein polymorphism.

Translated title of the contributionIdentification of Specific Plasma Proteins Determining the Agarose Gel Electrophoresis by the Immunosubtraction Technique
Original languageUndefined/Unknown
Pages (from-to)841-844
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Volume21
Issue number12
DOIs
Publication statusPublished - 1983

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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