Identification of sulfonylureas in serum by electrospray mass spectrometry

F. Magni, L. Marazzini, S. Pereira, L. Monti, M. Galli Kienle

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Identification of sulfonylureas in serum is important in the diagnosis of hypoglycemic crisis of unknown origin. Methods based on HPLC with UV or fluorescence detection may give false positive results. Mass spectrometry may successfully avoid this problem. The described method allows the simultaneous identification and quantification of tolbutamide, chlorpropamide, glibenclamide, and glipizide in human serum using one of the tested sulfonylureas as the internal standard. Serum purification is carried out by solidphase extraction with ENVI-C18 cartridges and samples are analyzed by liquid chromatographyelectrospray mass spectrometry. For all drugs, the limit of detection and the limit of quantification are about 2 and 10 ng/ml, respectively. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)136-141
Number of pages6
JournalAnalytical Biochemistry
Volume282
Issue number1
DOIs
Publication statusPublished - Jun 15 2000

Fingerprint

Mass spectrometry
Mass Spectrometry
Glipizide
Chlorpropamide
Tolbutamide
Glyburide
Serum
Hypoglycemic Agents
Purification
Fluorescence
Limit of Detection
Liquids
High Pressure Liquid Chromatography
Pharmaceutical Preparations

Keywords

  • Clinical chemistry
  • Electrospray
  • LC-ESI
  • Mass spectrometry
  • Sulfonylureas

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Identification of sulfonylureas in serum by electrospray mass spectrometry. / Magni, F.; Marazzini, L.; Pereira, S.; Monti, L.; Kienle, M. Galli.

In: Analytical Biochemistry, Vol. 282, No. 1, 15.06.2000, p. 136-141.

Research output: Contribution to journalArticle

Magni, F. ; Marazzini, L. ; Pereira, S. ; Monti, L. ; Kienle, M. Galli. / Identification of sulfonylureas in serum by electrospray mass spectrometry. In: Analytical Biochemistry. 2000 ; Vol. 282, No. 1. pp. 136-141.
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