Identification of tear lipocalin as a novel autoantigen target in Sjögren's syndrome

Riccardo Navone, Claudio Lunardi, Roberto Gerli, Elisa Tinazzi, Dimitri Peterlana, Caterina Bason, Roberto Corrocher, Antonio Puccetti

Research output: Contribution to journalArticle

Abstract

Sjögren's syndrome (SS) is an autoimmune disease characterized by lymphocytic infiltration and tissue damage mainly confined to the salivary and lacrimal glands, resulting in dryness of mouth and eyes. Since different epithelial cells of exocrine and non-exocrine tissues are primarily affected, an autoimmune reaction against antigens commonly expressed in epithelial cells is believed to play a pathogenic role. To identify novel autoantigen targets associated with the systemic involvement in SS, we screened a random peptide library with pooled IgG immunoglobulins derived from patients with primary SS. Among the identified peptides, one was recognized by the majority of patients' sera, but not by sera of normal donors and of patients with other autoimmune diseases. The peptide showed homology with an Epstein-Barr Virus (EBV) derived protein and with tear lipocalin, a protein highly expressed in tears and saliva, and with alpha-fodrin, a cytoskeleton protein considered an important autoantigen target in SS. Anti-peptide antibodies affinity purified from patients' sera recognize the viral protein, tear lipocalin and alpha-fodrin. Our findings suggest that EBV infection may be linked to the pathogenesis of SS and that tear lipocalin can be considered a novel and yet unidentified autoantigen in SS.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalJournal of Autoimmunity
Volume25
Issue number3
DOIs
Publication statusPublished - Nov 2005

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Keywords

  • Alpha-fodrin
  • Epstein-Barr virus
  • Lipocalin
  • Peptide library
  • Sjögren's syndrome

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Navone, R., Lunardi, C., Gerli, R., Tinazzi, E., Peterlana, D., Bason, C., Corrocher, R., & Puccetti, A. (2005). Identification of tear lipocalin as a novel autoantigen target in Sjögren's syndrome. Journal of Autoimmunity, 25(3), 229-234. https://doi.org/10.1016/j.jaut.2005.09.021