Abstract
Background: Several proteins have been identified as peanut allergens; among them, Ara h 1 (7S globulin) and Ara h 2 (2S globulin) are usually considered the major allergens. Objective: To identify the major allergens in a group of children selected for their specific pattern of immunoreactivity. Methods: We identified the dominant allergen by using (1) amino acid sequencing of the bands that show the strongest IgE immunoreactivity in 1-dimensional electrophoresis and immunoblotting and (2) specific animal IgGs raised against the dominant immunoreactive band to pinpoint the allergen(s) in peanut proteins separated by 2-dimensional electrophoresis and immunoblotting. To confirm these data, we further examined the peanut proteome using serum samples from the children with the unusual immunoreactivity. Results: We found a group of children with marked peanut allergy who are specifically sensitized to the basic subunit of Ara h 3 (11S globulin family). Conclusion: That the dominant immunoreactivity in these patients is in a basic subunit of Ara h 3 was unexpected, because previous studies had indicated that Ara h 3 was only a minor peanut allergen and that the identified allergenic epitopes occurred mainly in the acidic Ara h 3 subunit.
| Original language | English |
|---|---|
| Pages (from-to) | 262-266 |
| Number of pages | 5 |
| Journal | Annals of Allergy, Asthma and Immunology |
| Volume | 94 |
| Issue number | 2 |
| Publication status | Published - Feb 2005 |
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ASJC Scopus subject areas
- Immunology and Allergy
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Identification of the basic subunit of Ara h 3 as the major allergen in a group of children allergic to peanuts. / Restani, Patrizia; Ballabio, Cinzia; Corsini, Emanuela; Fiocchi, Alessandro; Isoardi, Patrizia; Magni, Chiara; Poiesi, Claudio; Terracciano, Luigi; Duranti, Marcello.
In: Annals of Allergy, Asthma and Immunology, Vol. 94, No. 2, 02.2005, p. 262-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of the basic subunit of Ara h 3 as the major allergen in a group of children allergic to peanuts
AU - Restani, Patrizia
AU - Ballabio, Cinzia
AU - Corsini, Emanuela
AU - Fiocchi, Alessandro
AU - Isoardi, Patrizia
AU - Magni, Chiara
AU - Poiesi, Claudio
AU - Terracciano, Luigi
AU - Duranti, Marcello
PY - 2005/2
Y1 - 2005/2
N2 - Background: Several proteins have been identified as peanut allergens; among them, Ara h 1 (7S globulin) and Ara h 2 (2S globulin) are usually considered the major allergens. Objective: To identify the major allergens in a group of children selected for their specific pattern of immunoreactivity. Methods: We identified the dominant allergen by using (1) amino acid sequencing of the bands that show the strongest IgE immunoreactivity in 1-dimensional electrophoresis and immunoblotting and (2) specific animal IgGs raised against the dominant immunoreactive band to pinpoint the allergen(s) in peanut proteins separated by 2-dimensional electrophoresis and immunoblotting. To confirm these data, we further examined the peanut proteome using serum samples from the children with the unusual immunoreactivity. Results: We found a group of children with marked peanut allergy who are specifically sensitized to the basic subunit of Ara h 3 (11S globulin family). Conclusion: That the dominant immunoreactivity in these patients is in a basic subunit of Ara h 3 was unexpected, because previous studies had indicated that Ara h 3 was only a minor peanut allergen and that the identified allergenic epitopes occurred mainly in the acidic Ara h 3 subunit.
AB - Background: Several proteins have been identified as peanut allergens; among them, Ara h 1 (7S globulin) and Ara h 2 (2S globulin) are usually considered the major allergens. Objective: To identify the major allergens in a group of children selected for their specific pattern of immunoreactivity. Methods: We identified the dominant allergen by using (1) amino acid sequencing of the bands that show the strongest IgE immunoreactivity in 1-dimensional electrophoresis and immunoblotting and (2) specific animal IgGs raised against the dominant immunoreactive band to pinpoint the allergen(s) in peanut proteins separated by 2-dimensional electrophoresis and immunoblotting. To confirm these data, we further examined the peanut proteome using serum samples from the children with the unusual immunoreactivity. Results: We found a group of children with marked peanut allergy who are specifically sensitized to the basic subunit of Ara h 3 (11S globulin family). Conclusion: That the dominant immunoreactivity in these patients is in a basic subunit of Ara h 3 was unexpected, because previous studies had indicated that Ara h 3 was only a minor peanut allergen and that the identified allergenic epitopes occurred mainly in the acidic Ara h 3 subunit.
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UR - http://www.scopus.com/inward/citedby.url?scp=13944258766&partnerID=8YFLogxK
M3 - Article
C2 - 15765743
AN - SCOPUS:13944258766
VL - 94
SP - 262
EP - 266
JO - Annals of Allergy, Asthma and Immunology
JF - Annals of Allergy, Asthma and Immunology
SN - 1081-1206
IS - 2
ER -