Identification of the bioactive peptide PEC-60 in brain

Å Norberg, S. Gruber, F. Angelucci, S. Renlund, H. Wadensten, S. Efendic, C. G. Östenson, H. Jörnvall, R. Sillard, A. A. Mathé

Research output: Contribution to journalArticle

Abstract

PEC-60 is a 60-residue peptide originally isolated from pig intestine. It inhibits glucose-induced insulin secretion from perfused pancreas in a hormonal manner and also has biological activity in the immune system. PEC-60-like immunoreactive material has been reported in catecholamine neurons of the central and peripheral nervous systems, but the peptide has not been identified from that material. We have now isolated PEC-60 from pig and rat brains with a method that combines column purification procedures with the specificity of a radioimmunoassay and the sensitivity of mass spectrometry to directly identify the peptide. The results show that PEC-60, like many other peptides, is expressed in the gastrointestinal tract and the central nervous system. The specific regional brain distribution and interaction with classical neurotransmitters raise the possibility that PEC-60 may play a role in the central nervous system disorders involving dopamine dysregulation.

Original languageEnglish
Pages (from-to)378-381
Number of pages4
JournalCellular and Molecular Life Sciences
Volume60
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Keywords

  • Brain
  • PEC-60
  • Pig
  • Radioimmunoassay
  • Rat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology

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    Norberg, Å., Gruber, S., Angelucci, F., Renlund, S., Wadensten, H., Efendic, S., Östenson, C. G., Jörnvall, H., Sillard, R., & Mathé, A. A. (2003). Identification of the bioactive peptide PEC-60 in brain. Cellular and Molecular Life Sciences, 60(2), 378-381. https://doi.org/10.1007/s000180300030