Identification of the CC chemokines TARC and macrophage inflammatory protein-1β as novel functional ligands for the CCR8 receptor

Giovanni Bernardini, Joseph Hedrick, Silvano Sozzani, Walter Luini, Gaia Spinetti, Mary Weiss, Satish Menon, Albert Zlotnik, Alberto Mantovani, Angela Santoni, Monica Napolitano

Research output: Contribution to journalArticle

Abstract

Chemokines are key molecules in directing leukocyte migration toward sites of inflammation. We have previously cloned a putative CC chemokine receptor gene, TER1, whose expression is restricted to lymphoid tissues and cell lines. Recently, this receptor has been shown to signal in response to the human CC chemokine I-309 and thus it has been renamed CCR8 according to the current nomenclature. In the present study, we report the identification of the CC chemokines thymus and activation-regulated cytokine (TARS) and macrophage inflammatory protein-1β (MIP-1β) as CCR8 ligands, as they induce chemotaxis in CCR8 Jurkat stable transfectants. Furthermore, we have generated a polyclonal antiserum that is able to recognize the CCR8 molecule in transfectant lysates. The pattern of CCR8 mRNA expression and the functional effects exerted by its ligand suggest that the triggering of this receptor may regulate multiple functions including activation, migration and proliferation of lymphoid cells.

Original languageEnglish
Pages (from-to)582-588
Number of pages7
JournalEuropean Journal of Immunology
Volume28
Issue number2
DOIs
Publication statusPublished - Feb 1998

Keywords

  • Chemokine receptor
  • Chemotaxis
  • Macrophage inflammatory protein-1β
  • Thymus
  • Thymus and activation-regulated cytokine

ASJC Scopus subject areas

  • Immunology

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