Identification of the human sex chromosome complement in polymorphonuclear leukocytes: A new technique

Research output: Contribution to journalArticle

Abstract

Diagnostic information about the female sex chromatin (drumstick) and the male chromatin (F-body) can be gained from the study of polymorphonuclear leukocytes stained with quinacrine hydrochloride. Cells from individuals with one or 2 Y chromosomes display one and 2 brightly fluorescent nuclear bodies, respectively. These structures are not present in normal females and in females carrying numerical or structural aberrations of the X chromosome. In such cases, however, the present staining technique is suitable to recognize the drumsticks and their size abnormalities. Furthermore, in subjects with 47,XXY (Klinefelter's syndrome), a proportion of cells display simultaneously both the presence of drumstick and F-body. Therefore, the quinacrine technique can be applied successfully to the study of polymorphonuclear leukocytes for the identification of the human sex complement. In fact, every sex chromosome constitution shows a specific nuclear pattern with regard to the number and size of drumsticks and F-bodies, which is of value in the screening of patients carrying sex chromosome abnormalities.

Original languageEnglish
Pages (from-to)88-93
Number of pages6
JournalThe Journal of Laboratory and Clinical Medicine
Volume78
Issue number1
Publication statusPublished - 1971

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

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